GB/T 4789.27-2008Microbiological examination of food hygiene - Examination of residue of antibiotics in fresh milk (English PDF)
食品卫生微生物学检验 鲜乳中抗生素残留检验
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 21, 2008
Implementation date
March 1, 2009
Scope
GB/T 4789.27-2008 is the English-translated version of 食品卫生微生物学检验 鲜乳中抗生素残留检验.
GB/T 4789.27-2008 is the Chinese standard "Microbiological examination of food hygiene - Examination of residue of antibiotics in fresh milk". Its scope clause reads: This standard specifies the test methods of antibiotic residues in milk.
The first method Streptococcus thermophilus inhibition Principle 2 Samples after 80 °C sterilization, add Streptococcus thermophilus bacteria. After the incubation period, Streptococcus thermophilus began to proliferate.
This time add The metabolic substrates 2,3,5-triphenyltetrazolium chloride (TTC), if the sample does not contain antibiotics or antibiotic concentration was below the detection limit, addicted Streptococcus thermophilus will continue to proliferate, reducing TTC become red substance.
Conversely, if the sample contains higher than the detection limit of antibacterial agents, the thermophilic Streptococcus is suppressed, the indicator TTC does not restore, maintain color.
3 equipment and materials In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows.
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Document preview — GB/T 4789.27-2008
National Standard of the People's Republic of China
- ICS
- 07.100.30
- Classification
- C 53
- Replacing
- GB/T 4789.27-2003
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3.1 Refrigerator
- 3.2 incubator
- 3.3 lid temperature water bath
- 3.4 Balance
- 3.5 sterile pipette
- 3.6 sterile tube
- 3.7 Thermometer
- 4.1 bacteria
- 4.2 sterilized skim milk
- 4.4 penicillin G reference solution
1 Scope
This standard specifies the test methods of antibiotic residues in milk.
The first method Streptococcus thermophilus inhibition
Principle 2
Samples after 80 °C sterilization, add Streptococcus thermophilus bacteria. After the incubation period, Streptococcus thermophilus began to proliferate. This time add
The metabolic substrates 2,3,5-triphenyltetrazolium chloride (TTC), if the sample does not contain antibiotics or antibiotic concentration was below the detection limit, addicted
Streptococcus thermophilus will continue to proliferate, reducing TTC become red substance. Conversely, if the sample contains higher than the detection limit of antibacterial agents, the thermophilic
Streptococcus is suppressed, the indicator TTC does not restore, maintain color.
3 equipment and materials
In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows.
3.1 Refrigerator
2 °C ~ 5 °C, -20 °C ~ -5 °C.
3.2 incubator
36 °C ± 1 °C.
3.3 lid temperature water bath
36 °C ± 1 °C, 80 °C ± 2 °C.
3.4 Balance
a sense of the amount of 0.1g, 0.001g.
3.5 sterile pipette
1mL (with 0.01mL scale), 10.0mL (with 0.1mL scale) or micro pipettes and tips.
3.6 sterile tube
18mm × 180mm.
3.7 Thermometer
0 °C ~ 100 °C.
3.8
Vortex Mixer.
4 strains, media and reagents
4.1 bacteria
Streptococcus thermophilus.
4.2 sterilized skim milk
see Section A. Chapter 1.
4.4 penicillin G reference solution
See Section A. 3.
5 test procedure
Antibiotic residues in milk testing procedures shown in Figure 1.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
Editions of GB/T 4789.27
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 4789.27-2008 | Microbiological examination of food hygiene - Examination of residue of antibiotics in fresh milk | current edition | Current |
| GB/T 4789.27-2003 | Microbiological examination of food hygiene - Examination of residue of antibiotics in fresh milk | previous edition | Superseded |
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