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GB/T 42880-2023Chemicals—Honeybee (Apis mellifera L.), chronic oral toxicity test (10-day feeding) (English PDF)

化学品 蜜蜂(Apis mellifera L.)慢性经口毒性试验(10天饲喂法)

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 6, 2023

Implementation date

December 1, 2023

Scope

GB/T 42880-2023 is the English-translated version of 化学品 蜜蜂(Apis mellifera L.)慢性经口毒性试验(10天饲喂法).

This document describes the chronic oral toxicity test on the honeybee, Apis mellifera L., by the 10-day feeding method: the information and parameters required on the test substance, the reference substances, the principle of the test, the description of the method, the test procedure, the quality assurance and quality control that apply, and the data and the report.

The document applies to the testing and evaluation of the chronic oral toxicity of chemicals to honeybees.

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Document preview — GB/T 42880-2023

National Standard of the People's Republic of China

ICS
13.300; 13.020
Classification
A 80

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • PrefaceI
  • Introduction
  • 1 Scope
  • 2 Normative reference documents
  • 3 Terms, definitions and abbreviations
  • 4 Test substance information
  • 5 Reference
  • 6 Test principle
  • 7 Method Description
  • 8 Test procedure
  • 9 Quality Assurance and Quality Control
  • 10 Data and Reports
  • Reference

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

Introduction

This document is a 10-day chronic oral toxicity test method for adult worker bees conducted under laboratory conditions.

Pollinating insects such as bees can be exposed to various pollutants over time by storing and eating contaminated food in the hive, or foraging in contaminated areas.

Plant protection products (PPP) or chemicals. In order to solve the above potential risks, adult worker bees can be fed and treated in the laboratory for 10 consecutive days. Food (sucrose solution) was used for chronic toxicity testing.

Before using this document to test a mixture and obtain relevant data for a specified regulatory purpose, it should be considered whether doing so (in order to and why) can provide sufficient test results to achieve the above purposes. When there is a regulation that specifically requires the mixture to be tested, there is no need to have this concern. Chemical Bee (Apismellifera L.) Chronic oral toxicity test (10-day feeding method)

1 Scope

This document describes the test substance information and parameters for the chemical bee (Apismellfera L.) chronic oral toxicity test (10-day feeding method)

Comparative materials, test principles, method descriptions, test procedures, quality assurance and quality control, data and reports.

This document is suitable for testing and evaluating the chronic oral toxicity of chemicals to bees (Apismellfera L.).

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document.

GB/T 21845 Chemical water solubility test

GB/T 21855 pH-related hydrolysis test of chemicals

GB/T 22052 Method for determining the vapor pressure-temperature relationship and initial decomposition temperature of liquids using a liquid vapor pressure meter

GB/T 22228 Static method for determination of vapor pressure of industrial chemical solids and liquids in the range of 10-1Pa to 105Pa

GB/T 22229 Determination of vapor pressure of industrial chemical solids and liquids in the range of 10-3Pa to 1Pa equity

3 Terms, definitions and abbreviations

3.1 Terms and definitions

The following terms and definitions apply to this document.

3.1.1 LCx

The concentration of test substance that causes the death of x% of the test organisms during a given exposure period compared with the control group.

3.1.2 LC50

After statistical calculation, the concentration of the test substance that causes the death of 50% of the test organisms at the end of the test is determined.

Note: The LC50 value in this document is expressed as the content of active ingredients or preparations per kilogram of food, mg (or µg)/kg.

3.1.3 LDD50

After statistical calculation, the dose of the test substance that causes the death of 50% of the test organisms at the end of the test is determined.

Note: The LDD50 value in this document is expressed as the daily dose of active ingredients or preparations consumed by each bee, µg (or ng)/(bee·d).

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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