GB/T 42367-2023Chemicals—Protozoan activated sludge inhibition test (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

Introduction

The principle of biological treatment in a domestic sewage treatment plant (STP) is to convert the organic matter contained in the influent sewage into microbial biomass.

The amount of matter, and then the microorganisms are separated from the liquid to obtain purified sewage, with the purpose of minimizing the organic load in the sewage and reducing the The content of nutrients such as ammonia nitrogen, nitrate and phosphate, while minimizing the amount of biological sludge. biophagocytosis of activated sludge A process played a role in promoting. In conventional wastewater treatment plants, the above-mentioned phagocytosis is usually dominated by ciliates, which feed on bacteria, Purify the sewage, further improve the transparency of the sewage, and reduce the organic load in the effluent.

The purpose of this method is to evaluate the impact of chemical substances on consumers in sewage treatment plants, which mainly consist of ciliated protozoa. composition.

This test is based on an international comparative study, and the relevant test methods have been validated in 5 laboratories. The above research used Five single-component organic compounds assumed to have non-polar or polar narcotic mode of action (MOA) were used as test substances, namely. 1-octylamine (CAS. 111-86-4), 3,5-Dichlorophenol (CAS. 591-35-5), Dimethyl Sulfoxide (CAS. 67-68-5), Diphenyl Ether (CAS. 101-84-8) and hexachlorophene (CAS. 70-30-4), the partition coefficients of these substances ranged from -1.36 to 6.91.

If this method is used to test the mixture and the data obtained are used for regulation, it is necessary to consider whether the test results are sufficient and valid.

1 Scope

This document describes the test principle, test substance information, reference substance, test preparation, test Procedures, Quality Assurance and Quality Control, Data Processing, Reporting.

This document applies to protozoan activated sludge inhibition tests for chemicals.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 21801 Rapid Biodegradability Respirometric Test of Chemicals

GB/T 21802 MITI Test for Improved Rapid Biodegradability of Chemicals (I)

GB/T 21803 Rapid biodegradability of chemicals DOC reduction test

GB/T 21831 Rapid Biodegradability Test of Chemicals by Closed Bottle Method

GB/T 21845 Water Solubility Test of Chemicals

GB/T 21852 Chemical partition coefficient (n-octanol-water) high performance liquid chromatography test

GB/T 21853 Partition coefficient of chemicals (n-octanol-water) shake flask test

GB/T 21856 Carbon dioxide generation test for rapid biodegradability of chemicals

GB/T 21857 OECD Screening Test for Improved Rapid Biodegradability of Chemicals

GB/T 22228 Static method for the determination of the vapor pressure of solid and liquid industrial chemicals in the range of 10-1Pa to 105Pa

GB/T 22229 Determination of vapor pressure level of solid and liquid vapor pressure of industrial chemicals in the range of 10-3Pa to 1Pa Equity

GB/T 27850 General rules for ready biodegradability of chemicals

EN15934 Sludge, treated biological waste, soil and waste Calculation of dry matter by determination of dry matter content or moisture

3 Terms and Definitions

The following terms and definitions apply to this document.