GB/T 41691-2022Chemicals—Honey bee larval single exposure toxicity test (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" drafted.

Introduction

This document describes a method for the acute toxicity test of honey bee larvae under laboratory conditions to the Organization for Economic Cooperation and Development (OECD) Chemical Testing Guideline No. 237 "Bee larvae toxicity test, single exposure" as the basis. The method of No.237 opens with a French Based on the relatively mature method developed, from 2005 to 2008, the chain test has been carried out in 7 European laboratories.

This method addresses the test requirements of the United States, Canada, and European countries, under laboratory conditions of a Tier 1 test strategy, feeding spiked feed to test the toxicity of chemicals to bee larvae.

The purpose of this method is to measure the value of a chemical (especially the active ingredient or formulation of a pesticide) by a single exposure of honeybee larvae 72h half-lethal dose (72h-LD50). The resulting data is used for bee population risk assessment. This method is a test for "Acute Oral Toxicity of Honeybees" Supplement to the adult bee test methods "Test" (GB/T 21812) and "Acute Contact Toxicity Test for Honeybees" (GB/T 21813).

In the risk assessment process, this method belongs to the lower-level test method.

Caution - Persons using this document should have practical experience in formal laboratory work. This document does not address all possible security issues question. It is the user's responsibility to take appropriate safety and health measures and to ensure compliance with the conditions stipulated by relevant national regulations.

1 Scope

This document specifies the test substance information, test principle, reference substance, test preparation, test inspection procedures, quality assurance and quality control, data and reporting.

This document is suitable for testing the toxicity of water-soluble, non-volatile chemicals to a single exposure to honeybee larvae. This document does not apply to volatile chemicals.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document.

GB/T 21812 Chemical Bee Acute Oral Toxicity Test

GB/T 21813 Chemical Bee Acute Contact Toxicity Test

GB/T 21845 Chemical Water Solubility Test

GB/T 21852 Chemical Partition Coefficient (n-Octanol-Water) High Performance Liquid Chromatography Test

GB/T 21853 Chemical Partition Coefficient (n-Octanol-Water) Shake Flask Test

GB/T 21855 Hydrolysis test of chemicals related to pH

GB/T 22052 Method for Determining Liquid Vapor Pressure Temperature Relationship and Initial Decomposition Temperature with Liquid Vapor Manometer

GB/T 22228 Static method for the determination of the vapor pressure of solids and liquids in industrial chemicals in the range of 10-1Pa to 105Pa

GB/T 22229 Determination of the vapor pressure of solids and liquids of industrial chemicals in the range of 10-3Pa to 1Pa Vapor pressure level balance method

OECD Chemical Testing Guideline No.104 Vapor Pressure (Vapour Pressure)

3 Terms and Definitions

Terms and definitions defined in GB/T 21812 and GB/T 21813 apply to this document.

4 Test substance information

The following information on the test substance is helpful for trial design.

a) Solubility in water determined according to GB/T 21845;

b) Solubility in organic solvents;

c) Measured in accordance with GB/T 22052, GB/T 22228, GB/T 22229 or OECD Chemical Testing Guideline No.104