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GB/T 39914-2021Variety genuineness and purity testing of main crops with SSR markers—Maize (English PDF)

主要农作物品种真实性和纯度SSR分子标记检测玉米

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

April 30, 2021

Implementation date

November 1, 2021

Scope

GB/T 39914-2021 is the English-translated version of 主要农作物品种真实性和纯度SSR分子标记检测玉米.

GB/T 39914-2021 is the part of the Chinese series on testing crop varieties with SSR markers that covers maize.

It gives the terms, definitions and abbreviations, the general rules, the detection plan - the primers, the detection platform, the samples and the test conditions - the equipment, reagents and solutions, the test procedure and the results report.

It applies to verifying the genuineness of a maize variety and identifying its purity, and not to essentially derived varieties nor to genetically modified ones.

Seed companies, seed control stations and testing laboratories use it. Issued on 30 April 2021, it has applied since 1 November 2021.

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Document preview — GB/T 39914-2021

National Standard of the People's Republic of China

ICS: 65.020.20
Classification: B 21

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Foreword
1 Scope
2 Normative references
3 Terms, definitions and abbreviations
3.1 Terms and definitions
3.2 Abbreviations
4 General
5 Detection plan
5.1 General
5.2 Primer
5.3 Detection platform
5.4 Sample
5.5 Test condition
6 Equipment, reagents and solution preparation
6.1 Instruments and equipment
6.2 Reagent
6.3 Solution preparation
7 Authenticity test procedure
7.1 Primer synthesis
7.2 DNA extraction
7.3 PCR amplification
7.4 Separation of amplified products
7.5 Data analysis
8 Variety purity testing procedures
8.1 DNA extraction
8.2 Primer screening and synthesis
8.3 PCR amplification
8.4 Separation of amplified products
8.5 Data analysis
9 Calculation and presentation of results
9.1 Verification of authenticity
9.2 Determination of purity
10 Results report
10.1 Verification of authenticity
10.2 Purity determination
Appendix A (Normative Appendix) Solution Preparation
Appendix B (informative appendix) Allele amplified fragment information

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the detection principles, detection schemes, and inspections of the SSR molecular markers for the authenticity and purity of maize (Zeamays L.) varieties. Test procedures and results report.

This standard applies to the authenticity verification and authenticity identification of corn varieties, and does not apply to substantive derived varieties (EDV) and genetically modified varieties. Variety identification.

This standard applies to the purity determination of maize single-cross varieties, and the purity determination of inbred lines, double-cross varieties and triple-cross varieties can be implemented by reference.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 3543.1 General rules for inspection of crop seeds

GB/T 3543.2 Crop Seed Inspection Regulation Sample

GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms, definitions and abbreviations

3.1 Terms and definitions

The following terms and definitions apply to this document.

3.1.1 Variety verification

Compared with the standard sample of the corresponding variety name, the test confirms whether the labeling of the variety name of the sample for inspection is worthy of the name.

3.1.2 Variety identification

After SSR molecular marker detection and through the SSR fingerprint data comparison platform (3.1.4) of the approved varieties, the screening and comparison are carried out to determine the authenticity of the samples for inspection. The name of the actual variety.

3.1.3 Standard sample

The physical seed samples that have been recognized as representative of the characteristics of the approved variety and kept by the designated agency of the state.

3.1.4 SSR fingerprint data comparison platform SSRfingerprintblastplatform

The standardized method of SSR molecular markers is used to detect the alleles of the variety standard samples, and the computer database technology and A search and comparison carrier for molecular data information of approved varieties constructed by network information technology.

3.1.5 Reference sample

It is used to calibrate the sample whose SSR allele has defined the size of the amplified product fragment.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 18 pages — is available in the English PDF.

Referenced standards

Normative references

Similar standards

GB 19169-2026|GB 19170-2026|GB 19171-2026|GB 19172-2026|GB 4404.2-2026|GB/T 17315-2026

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