GB/T 35909-2018Detection of mycoplasma hyopneumoniae using PCR method (English PDF)
猪肺炎支原体PCR检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
February 6, 2018
Implementation date
September 1, 2018
Scope
GB/T 35909-2018 is the English-translated version of 猪肺炎支原体PCR检测方法.
GB/T 35909-2018 sets out a PCR method for detecting Mycoplasma hyopneumoniae, the main pathogen of mycoplasmal pneumonia of swine, also known as swine asthma.
It is intended for the rapid detection of the organism's nucleic acid in laboratory clinical samples from pigs, such as nasal swabs, lung tissue and bronchoalveolar lavage fluid, and in bacterial cultures.
The standard relies on GB 19489 for laboratory biosafety, GB/T 27401 for laboratory quality control and GB/T 6682 for laboratory water. It defines the pathogen and the abbreviations used in the method, among them PCR, the PBS, TAE and TBE buffers, Taq DNA polymerase, dNTPs and the colour change unit (CCU).
It is a working method for veterinary diagnostic laboratories. It was issued on 6 February 2018 and has applied since 1 September 2018.
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Document preview — GB/T 35909-2018
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions and abbreviations
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
1 Scope
This standard specifies the method of PCR detection of Mycoplasma hyopneumoniae.
This standard applies to laboratory clinical samples of pigs (swine nasal swabs, lung and bronchoalveolar lavage fluid), bacterial culture and animal production Rapid detection of Mycoplasma hyopneumoniae nucleic acid in […]
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB 19489 laboratory biosafety common requirements
GB/T 27401 Laboratory Quality Control Animal Quarantine
Diseases and disease harmless animal handling technical specifications Nongyifa [2017] No. 25
Veterinary Laboratory Biosafety Regulations (People's Republic of China Ministry of Agriculture Bulletin No. 302)
3 Terms and definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1 Mycoplasma hyopneumoniae; MHP
Mycoplasma Mycoplasma is a member of the Mycoplasma pneumoniae (MPS) major pathogens.
Note: Mycoplasma hominis also known as swine asthma, is a chronic infection of the respiratory tract, causing serious damage to the pig industry.
3.2 Abbreviations
The following abbreviations apply to this document.
PCR polymerase chain reaction (polymerase chain reaction)
PBS phosphate buffer (phosphate-buffered saline buffer)
TAE TAE electrophoresis buffer (tris-acetate-ethylenediaminetetraacetic acid buffer)
TBE TBE electrophoresis buffer (tris-borate-ethylenediaminetetraacetic acid buffer)
Taq polymerase Taq DNA polymerase (Taq DNA polymerase)
dNTPs deoxyribonucleoside triphosphates
DNA deoxyribonucleic acid
CCU color change unit (color change unit)
MPS Mycoplasma pneumonia of swine
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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Related Standards
GB 19489-2008 — Laboratories - General requirements for biosafety
GB/T 27401-2026 — Criterion on the quality control of laboratories - Veterinary testing
GB/T 6682-2008 — Water for analytical laboratory use - Specification and test methods
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GB/T 35909-2018
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