GB/T 35906-2018Indirect ELISA to detect antibody against classical swine fever virus (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

Introduction

Bovine viral diarrhea/mucosal disease virus infection in cattle more serious, and occasionally reports of natural infection in pigs. In the field, cattle are viral Diarrhea/mucosal disease Viruses are usually not pathogenic to postnatal pigs and are pathogenic only to the piglets. Although bovine viral diarrhea/mucosal disease virus can be In pigs spread, but this spread is very limited. In the absence of a new source of introduction, the herd has been present in cattle herds Diarrhea/mucosal disease virus will be gradually cleared, the virus's infectious chain will be terminated. Cattle bovine viral diarrhea/mucosal disease virus is a pig infected with cattle disease The main source of virulent diarrhea/mucosal disease virus, mainly cow polyculture, or the use of cattle that contaminate bovine viral diarrhea/mucosal disease virus Source material related. The main hog breeding in our country mainly for large and medium-sized farms, there is very little polyculture condition, it will not lead to cow disease The spread of toxic diarrhea/mucosal disease virus in swine herds. In addition, the raw materials used in the production of pig vaccines are all cattle Viral diarrhea/mucosal disease virus and other exogenous virus detection, therefore, the vaccine production process to improve and also completely cut off bovine viral diarrhea/mucosa Virus transmission in pigs. Finally, the immune rate of hog cholera vaccine in China is close to 100%. The classical swine fever virus and bovine viral diarrhea / Mucosal virus belong to the same virus, there is some cross-protection on immunity. Bovine viral diarrhea/mucosal disease antibody positive Very little energy. Therefore, this standard is mainly aimed at swine fever vaccine in swine herd immunized after antibody testing, not for individual swine fever antibody And bovine viral diarrhea/mucosal disease antibody detection.

1 Scope

This standard specifies the indirect ELISA detection of classical swine fever antibody.

This standard applies to swine fever antibody testing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies Pieces. For undated references, the latest edition (including all amendments) applies to this document.

Veterinary Laboratory Biosafety Management Practices (Ministry of Agriculture Bulletin No. 302)

3 Abbreviations

The following abbreviations apply to this document.

CSFV. classical swine fever virus

ELISA. Enzyme-Linked Immunosorbent Assay

HRP. horseradish peroxidase

OD. Optical Density

4 Reagent

4.1 Classical swine fever virus E2 protein. see Appendix A.

4.2 standard positive serum. piglet vaccine immunization piglet preparation, fluorescent antibody and virus neutralization test neutralization antibody titer >= 1. 20480.

4.3 standard negative serum. no maternal antibodies, piglets not vaccinated with classical swine fever vaccine serum, fluorescent antibody and virus neutralizing test antibody was detected as negative.

4.4 enzyme conjugate. commercial HRP-labeled anti-swine IgG antibody, working concentration with reference to product manual.

4.5 Coating solution. see Appendix B in B.1.

4.6 Phosphate buffer. See B.2.

4.7 Blocking solution. see B.3.

4.8 1 × washing liquid. see B.4.

4.9 Dilutions. See B.5.

4.10 Substrate Solution A. See B.6.

4.11 Substrate B. See B.7.

4.12 Stop Solution. See B.8.

4.13 Commercial kit. choose the same commercial kit.