GB/T 35900.1-2018Animal influenza detection—Part 1:Method of real-time RT-PCR for the detection of H1 subtype influenza virus (English PDF)

Foreword

GB/T 35900 Animal Influenza Test is currently divided into 3 parts.

--- Part 1. Fluorescent RT-PCR detection method for H1 subtype influenza virus nucleic acid;

--- Part 2. Detection of nucleic acid fluorescence of H3 subtype influenza virus by RT-PCR;

--- Part 3. Double-fluorescence RT-PCR detection method for H1 and H3 subtype influenza virus nucleic acids.

This part is part 1 of GB/T 35900.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This part of GB/T 35900 specifies a fluorescent RT-PCR method for the detection of H1 subtype influenza virus nucleic acids.

This section applies to the detection of H1 subtype influenza virus nucleic acids in swine and poultry and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest version (including all amendments) applies to this document.

GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

GB/T 19438.1-2004 Avian influenza virus universal fluorescence RT-PCR detection method

GB 19489 General Requirements for Laboratory Biosafety

3 Abbreviations

The following abbreviations apply to this document.

Fluorescence RT-PCR Real-time reverse transcription polymerase chain reaction

Ct (or Cp) value The number of cycles experienced by the amount of fluorescence signal in each reaction tube reaching the set threshold (cycle threshold, or crossing point)

cRNA complement RNA

DEPC diethylpyrocarbonate

FAM carboxyfluorescein

LNA locked nucleic acid

PBS phosphate buffer solution

RNA ribonucleic acid

TAMRA carboxytetramethylrhodamine

4 Reagents and materials