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GB/T 34740-2017Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay (English PDF)

动物狂犬病直接免疫荧光诊断方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

November 1, 2017

Implementation date

May 1, 2018

Scope

GB/T 34740-2017 is the English-translated version of 动物狂犬病直接免疫荧光诊断方法.

GB/T 34740-2017 is the Chinese standard "Diagnostic methods for animal rabies diagnosis with direct immunofluorescence assay".

Its scope clause reads: This standard specifies the materials and reagents, equipment and equipment, methods of protection, animal brain Sample collection, transportation and preservation, experimental operation and microscopic observation and determination of results.

This standard applies to animal rabies etiology diagnosis. Its clauses include abbreviations; materials and reagents; equipment and equipment.

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Document preview — GB/T 34740-2017

National Standard of the People's Republic of China

ICS: 11.220
Classification: B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

1 Scope
2 Normative references
3 Abbreviations
4 materials and reagents
5 Equipment and equipment

1 Scope

This standard specifies the materials and reagents, equipment and equipment, methods of protection, animal brain

Sample collection, transportation and preservation, experimental operation and microscopic observation and determination of results.

This standard applies to animal rabies etiology diagnosis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

Laboratory use specifications and test methods for analysis GB/T 6682-2008

3 Abbreviations

The following abbreviations apply to this document.

DFA. Direct Immunofluorescence Assay

FITC Fluorescein Isothiocyanate

PBS. Phosphate-Buffered Saline

4 materials and reagents

Unless otherwise specified, only analytical reagents are used.

4.1

water, GB/T 6682-2008, two water.

4.2

rabies positive control brain tissue (see A.1).

4.3

Rabies negative control brain tissue (see A.2).

4.4

FITC-labeled anti-rabies virus antibody (commercial fluorescent antibody) should be anti-rabies virus nucleoprotein monoclonal antibody or

Prepared from polyclonal antibodies, diluted with PBS (0.01mol/L, pH7.4, see B.1) into a working solution, now with the current service.

4.6

Stationary fluid (80% acetone solution, see B.3).

Warning --- acetone flammable, harmful to health 4.7 PBS (0.01 mol/L, pH 7.4, see B.1).

5 Equipment and equipment

5.1 necropsy sampling and protective equipment

Scalpel, scissors, forceps, tweezers, latex gloves, goggles.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Cited by

GB/T 18639-2023Diagnostic techniques for rabies

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