GB/T 34739-2017Detecting technique of neutralization antibody against rabies virus in animals (English PDF)
动物狂犬病病毒中和抗体检测技术
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 1, 2017
Implementation date
May 1, 2018
Scope
GB/T 34739-2017 is the English-translated version of 动物狂犬病病毒中和抗体检测技术.
GB/T 34739-2017 is the Chinese standard "Detecting technique of neutralization antibody against rabies virus in animals".
Its scope clause reads: This standard specifies the serum rabies serum neutralizing antibody for quantitative determination of fluorescent antibody virus neutralization test (FAVN) serum samples Product collection, experimental methods of operation and the results of the decision.
This standard applies to animal rabies quantitative immune effects, population immunization coverage and rabies epidemic risk assessment. Its clauses include abbreviations; reagents and materials.
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Document preview — GB/T 34739-2017
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Abbreviations
- 4 Reagents and materials
1 Scope
This standard specifies the serum rabies serum neutralizing antibody for quantitative determination of fluorescent antibody virus neutralization test (FAVN) serum samples
Product collection, experimental methods of operation and the results of the decision.
This standard applies to animal rabies quantitative immune effects, population immunization coverage and rabies epidemic risk assessment.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Laboratory use specifications and test methods for analysis GB/T 6682-2008
GB/T 19489-2008 Laboratory biosafety general requirements
3 Abbreviations
The following abbreviations apply to this document.
ATV. Antibiotic-Trypsin-Versene
BHK-21. Baby Hamster Kidney-21
BSL-2. Biosafety Laboratory Level 2
CVS. Rabies standard challenge strain (ChalengeVirusStandard)
FAVN. Fluorescent Antibody Virus Neutralization Test
FITC. Fluorescein Isothiocyanate
MEM. Basal Medium (Eagle's Minimum Essential Medium)
PBS. Phosphate-Buffered Saline
TCID50. Half cell infection (Median Tissue Culture Infective Dose)
4 Reagents and materials
Unless otherwise specified, only analytical reagents are used.
4.1 water
GB/T 6682-2008, two water.
4.2
commercial rabies negative standard dog serum.
4.3
commercial rabies positive standard dog serum, diluted to 0.5IU/mL before use.
4.4
FITC-labeled anti-rabies virus antibody, diluted with PBS (0.01mol/L, pH7.4, see A.1) into a working solution, now with the current service.
4.5
MEM (see A.2).
4.6
Cell culture with antibiotic solution (see A.3).
4.7
Antibiotic-trypsin-ethylenediaminetetraacetic acid (ATV) solution (see A.4).
4.8
Fixative .80% Acetone (see A.5).
Warning --- acetone flammable, harmful to health.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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