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GB/T 34729-2017Blocking ELISA method to detect antibody against classical swine fever virus (English PDF)

猪瘟病毒阻断ELISA抗体检测方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

November 1, 2017

Implementation date

May 1, 2018

Scope

GB/T 34729-2017 is the English-translated version of 猪瘟病毒阻断ELISA抗体检测方法.

GB/T 34729-2017 is the Chinese standard "Blocking ELISA method to detect antibody against classical swine fever virus". Its scope clause reads: This standard specifies the detection of swine fever virus antibody blocking ELISA method.

This standard applies to swine fever antibody testing, but can not distinguish vaccine immunogens and wild-type antibodies. Its clauses include abbreviations; reagent; equipment and equipment.

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Document preview — GB/T 34729-2017

National Standard of the People's Republic of China

ICS: 11.220
Classification: B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

1 Scope
2 Normative references
3 Abbreviations
4 reagent
5 Equipment and equipment

1 Scope

This standard specifies the detection of swine fever virus antibody blocking ELISA method.

This standard applies to swine fever antibody testing, but can not distinguish vaccine immunogens and wild-type antibodies.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

Veterinary Laboratories Biosafety Technical Management Specification Ministry of Agriculture Bulletin No. 302

3 Abbreviations

The following abbreviations apply to this document.

CSFV Classical swine fever virus

ELISA. Enzyme-Linked Immunosorbent Assay

HRP. Horseradish Peroxidase

OD. Optical Density (OpticalDensity)

4 reagent

4.1

Classical swine fever virus E2 protein.

4.2 Enzyme conjugates

See Appendix B.

4.3 standard positive serum

Swine fever vaccine immunization piglets prepared by fluorescence antibody virus neutralization test was positive.

4.4 Standard Negative Serum

The serum of piglets without maternal antibodies and non-immunized swine fever vaccine was negative by fluorescent antibody virus neutralization test.

4.5 Coating liquid

See C.1.

4.6 Phosphate buffer

See C.2.

4.7

Blocking fluid See C.3.

4.9

Dilutions See C.5.

4.10

Substrate Solution A. See C.6.

4.11 Substrate B

See C.7.

4.11 Stop solution

See C.8.

4.12 Commercial kits

Can choose the same commercial kits.

5 Equipment and equipment

5.1

37 °C incubator.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Cited by

GB/T 16551-2020Diagnostic techniques for classical swine fever

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