GB/T 32948-2016Detection of coproantigen of canidae infected Echinococcus granulosus (E.g) by sandwich enzyme-linked immunosorbent assay (English PDF)
犬科动物感染细粒棘球绦虫粪抗原的抗体夹心酶联免疫吸附试验检测技术
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
August 29, 2016
Implementation date
March 1, 2017
Scope
GB/T 32948-2016 is the English-translated version of 犬科动物感染细粒棘球绦虫粪抗原的抗体夹心酶联免疫吸附试验检测技术.
GB/T 32948-2016 is the Chinese standard "Detection of coproantigen of canidae infected Echinococcus granulosus (E.g) by sandwich enzyme-linked immunosorbent assay".
Its scope clause reads: This standard specifies the sandwich enzyme-linked immunosorbent assay (SandwichELISA) Claim. This standard is applicable to the diagnosis, quarantine, efficacy assessment and related epidemiology of terminal dendritic echinococcosis infection (canine) survey.
Its clauses include normative reference documents; terms and definitions; abbreviations.
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Document preview — GB/T 32948-2016
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 normative reference documents
- 3 terms and definitions
- 4 abbreviations
1 Scope
This standard specifies the sandwich enzyme-linked immunosorbent assay (SandwichELISA)
Claim.
This standard is applicable to the diagnosis, quarantine, efficacy assessment and related epidemiology of terminal dendritic echinococcosis infection (canine) survey.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General requirements for laboratory biosecurity
3 terms and definitions
The following terms and definitions apply to this document.
3.1 Echinococcus granulosus fecalantigenofEchinococcusgranulosus
Echinococcus granulosus parasitic in the dog's gut, with the feces together with the discharge of insects, metabolites and fall off the pregnancy.
3.2 Antibody sandwich
Polyclonal antibodies and antigen immune binding, antigen and monoclonal antibodies with specific binding, the formation of antibody - antigen - antibody "sandwich"
Type complexes.
3.3 Echinococcus granulosus Eggs
The surface antigen (EgsfAg) obtained by the action of 1% Triton × 100 in Echinococcus granulosus adults.
3.4 Rabbit anti-EgsfAg immunoglobulin Gtherabbit'simmunoglobulinGagainstEgsfAg
Purified anti-EgsfAg polyclonal rabbit antibody IgG (protein content of 11.12mg/mL, antibody titer of 1.160000 or more).
3.5 Anti-EgsfAg mouse monoclonal antibody mousemonoclonalantibodyagainstEgsfAg
Anti-EgsfAg mouse monoclonal antibody (protein content of 14.4mg/mL, antibody titer of 1. 800000 or more).
4 abbreviations
The following abbreviations apply to this document.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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