GB/T 32757-2016Molluscs karyotype analysis (English PDF)
贝类染色体组型分析
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
June 14, 2016
Implementation date
January 1, 2017
Scope
GB/T 32757-2016 is the English-translated version of 贝类染色体组型分析.
GB/T 32757-2016 is the Chinese standard "Molluscs karyotype analysis". Its scope clause reads: This standard gives the method, the apparatus and the equipment, the preparation and the group analysis method of the shell specimen.
This standard applies to shellfish genome analysis. Its clauses include normative reference documents; terms and definitions; principle.
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Document preview — GB/T 32757-2016
National Standard of the People's Republic of China
- ICS
- 65.150
- Classification
- B 50
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 normative reference documents
- 3 terms and definitions
- 4 principle
1 Scope
This standard gives the method, the apparatus and the equipment, the preparation and the group analysis method of the shell specimen.
This standard applies to shellfish genome analysis.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
Breeding of aquaculture species - Part 12. Analysis of chromosome composition
3 terms and definitions
GB/T 18654.12 Definitions and the following terms and definitions apply to this document.
3.1 Embryo method
Methods of analyzing chromosomes by using shellfish embryos as materials.
3.2 Trout larvae method
The method of analyzing the genome of the larvae using the shellfish larvae.
3.3 Adult gill tissue method
The method of using the shellfish gill tissue as material to analyze its chromosome type.
3.4 Heterotypic chromosome
A pair of homologous chromosomes of different shapes.
3.5 Secondary constriction
Some of the chromosomal DNA loosened to form a constricted portion of the nucleolar organizer region.
3.6 With the body satelite, trabant
Through the secondary constriction associated with the main part of the chromosome, located at the end of the chromosome, round or cylindrical chromosome fragments.
4 principle
The use of PHA to increase the cell division phase, colchicine damage the cells in the spindle wire, the same method used to prepare chromosome glass slide
This, to obtain a complete number, clear shape of the chromosome image for chromosome analysis.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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