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GB/T 30737-2014Determination of marine photosynthetic picoplankton - flow cytometry (English PDF)

海洋微微型光合浮游生物的测定 流式细胞测定法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

March 27, 2014

Implementation date

October 1, 2014

Scope

GB/T 30737-2014 is the English-translated version of 海洋微微型光合浮游生物的测定 流式细胞测定法.

GB/T 30737-2014 is the Chinese standard "Determination of marine photosynthetic picoplankton - flow cytometry". Its scope clause reads: This standard specifies the ocean pico plankton photosynthesis Central Prochlorococcus, Synechococcus and eukaryotic algal cells slightly streaming fine amount Cellular assays.

This standard applies to the three major groups of the number of cells was measured by flow cytometry ocean pico photosynthetic plankton. Its clauses include terms and definitions.

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Document preview — GB/T 30737-2014

National Standard of the People's Republic of China

ICS
07.060
Classification
A 45

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 principle of the method
  • 6 Reagent
  • 7 Sample collection and processing
  • 8 measuring step
  • 9 Analysis and Calculation

1 Scope

This standard specifies the ocean pico plankton photosynthesis Central Prochlorococcus, Synechococcus and eukaryotic algal cells slightly streaming fine amount

Cellular assays.

This standard applies to the three major groups of the number of cells was measured by flow cytometry ocean pico photosynthetic plankton.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein

Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 12763.6-2007 oceanographic survey - Part 6. Marine Biological Survey

3 Terms and Definitions

GB/T 12763.6-2007 and as defined in the following terms and definitions apply to this document.

3.1 Plankton

Lack of well-developed organ movement, no or only weak athletic ability, suspended in the water layer, often with the water moving creatures.

NOTE. rewrite GB/T 12763.6-2007, definitions 3.9.

3.2 Picoplankton

Range of particle size 0.2µm ~ 2µm plankton.

Note 1. picoplankton is not a strictly biological concept, but a concept of ecology.

Note 2. rewrite GB/T 12763.6-2007, definitions 3.9.

3.3 Pico photosynthetic plankton

Can photosynthetic picoplankton.

NOTE. pico plankton photosynthesis and autotrophic and heterotrophic include two types of self-support type predominant. Autotrophic photosynthetic plankton by a slight

The three main groups. the Prochlorococcus, Synechococcus and pico eukaryotic algae. Heterotrophic photosynthetic plankton slightly mainly Anoxygenic photosynthetic fine

Bacteria (anoxygenicphototrophicbacteria, APB).

3.4

Prochlorococcus Prochlorococcusspp;. Pro.

Blue bacteria (Cyanobacteria) in a genus of prokaryotes, the particle size is generally about 0.6µm, its main feature is the pigment divinyl

Group chlorophyll (DiVinyl-Chlorophyl, DVChl).

3.5

Synechococcus Synechococcusspp;. Syn.

A genus of cyanobacteria, the prokaryotes, the particle size is generally about 1µm, in addition to containing chlorophyll a (Chlorophyla), its main […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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