GB/T 28940-2012Sampling procedure of vector infected by pathogens - Rodent (English PDF)
病媒生物感染病原体采样规程 鼠类
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 20, 2012
Implementation date
May 1, 2013
Scope
GB/T 28940-2012 is the English-translated version of 病媒生物感染病原体采样规程 鼠类.
GB/T 28940-2012 is the Chinese standard "Sampling procedure of vector infected by pathogens - Rodent". Its scope clause reads: This standard specifies the security organs of rats and mice and blood sampling, sample preservation methods and the whole process.
This standard applies to infected or potentially infected rodents live pathogen sampling and preservation. Its clauses include reagents and materials; laboratory supplies.
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Document preview — GB/T 28940-2012
National Standard of the People's Republic of China
- ICS
- 11.020
- Classification
- C 05
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Reagents and materials
- 4 laboratory supplies
1 Scope
This standard specifies the security organs of rats and mice and blood sampling, sample preservation methods and the whole process.
This standard applies to infected or potentially infected rodents live pathogen sampling and preservation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria
3 Reagents and materials
3.1 Disinfectants
2% iodine, 75% alcohol.
3.2 Anticoagulants
Ethylenediaminetetraacetic acid (EDTA), oxalate mixture, heparin, sodium citrate, sodium fluoride, potassium oxalate. Preparation and dosage see Appendix A.
3.3 tissue, organ preservation solution
Neutral 20% Glycerol, 50% glycerol phosphate buffer, tissue preservation solution, RNAlater, storage solution. Preparation and dosage see sidebar
Record B.
3.4 anesthetics
Diethyl ether, chloroform.
3.5 Other
Liquid nitrogen, dry ice.
4 laboratory supplies
4.1 experimental equipment
Biological safety cabinets, -80 °C low temperature refrigerators, autoclaves, liquid nitrogen tanks, centrifuges, pipettes, covered enamel pan, once No. 3 to No. 5
Of syringe needles, tweezers straight head ophthalmology, ophthalmic scissors, large forceps, straws, plates, vials, needles and thread, rat bags, mouse fixing plate, mouse cassette, incinerator.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
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