GB/T 27540-2011Method of the real-time RT-PCR for the detection of classical swine fever virus (English PDF)

1 Scope

This standard specifies the swine fever virus (Classicalswinefevervirus, CSFV) real-time fluorescent RT-PCR detection method.

This standard applies to the diagnosis and monitoring of classical swine fever for live pigs and their organs, blood, excrement, and cell culture CSFV nuclear

Acid test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein

Member. For undated references, the latest edition (including any amendments) applies to this document.

People's Republic of China Ministry of Agriculture Bulletin No. 302 veterinary laboratory biosafety management practices

3 Abbreviations

The following abbreviations apply to this document.

CSFV. classical swine fever virus (Classicalswinefevervirus)

Ct values. reach the threshold number of cycles (cyclethreshold)

DEPC. diethyl pyrocarbonate (diethylpyrocarbonate)

HSTaq enzyme. TaqDNA hot start polymerase (HSTaqDNApolymerase)

RNA. ribonucleic acid (ribonucleicacid)

Fluorescent RT-PCR. reverse transcriptase polymerase chain reaction fluorescence (realtimefluorescentquantitativereverse transcriptionpolymerasechainreaction)

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade; all reagents were used without RNA enzyme dispensing container.