GB/T 27521-2011RT-PCR assay for swine influenza virus nucleic acid (English PDF)
猪流感病毒核酸RT-PCR检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 21, 2011
Implementation date
March 1, 2012
Scope
GB/T 27521-2011 is the English-translated version of 猪流感病毒核酸RT-PCR检测方法.
GB/T 27521-2011 is the Chinese standard "RT-PCR assay for swine influenza virus nucleic acid". Its scope clause reads: This standard specifies the technical requirements for swine influenza virus nucleic acid by RT-PCR methods.
RT-PCR detection method specified in this standard for the detection of swine lung tissue, nasal and tracheal secretions and culture of these samples were collected It was the swine flu virus nucleic acid. Its clauses include acronyms; instrument; reagents.
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Document preview — GB/T 27521-2011
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B 41
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Acronyms
- 3 Instrument
- 4 Reagents
1 Scope
This standard specifies the technical requirements for swine influenza virus nucleic acid by RT-PCR methods.
RT-PCR detection method specified in this standard for the detection of swine lung tissue, nasal and tracheal secretions and culture of these samples were collected
It was the swine flu virus nucleic acid.
2 Acronyms
The following abbreviations apply to this document.
DEPC. diethyl pyrocarbonate (diethypyrocarbonate) dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate)
EB. ethidium bromide (ethidiumbromide)
M-MLVRT. Moloney murine leukemia virus reverse transcriptase (moloneymurineleukemiavirusreversetran- scriptase)
PCR. polymerase chain reaction (polymerasechainreaction)
RNA. ribonucleic acid (ribonucleicacid)
RNAaseinhibitor. RNA inhibitors
RT-PCR. reverse transcription - polymerase chain reaction (reverse-transcriptionpolymerasechainreaction)
SPF. specific pathogen free (specificpathogenfree)
TaqDNApolymerase. TaqDNA polymerase
3 Instrument
3.1
PCR instrument.
3.2
low-speed desktop centrifuge.
3.3
electrophoresis.
3.4
electrophoresis tank.
3.5
refrigerator.
3.6
gel imaging system.
3.7
micropipette.
3.8
water bath.
4 Reagents
4.1
LSTRIzolRNA RNA extraction reagents or other commercially available extraction reagent.
4.2
of chloroform, isopropanol, ethanol.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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