GB/T 2441.2-2010Determination of urea - Part 2: Biuret content - Spectrophotometric method (English PDF)
尿素的测定方法 第2部分：缩二脲含量 分光光度法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
June 30, 2010
Implementation date
January 1, 2011
Scope
GB/T 2441.2-2010 is the English-translated version of 尿素的测定方法 第2部分：缩二脲含量 分光光度法.
GB/T 2441.2-2010 is the Chinese standard "Determination of urea - Part 2: Biuret content - Spectrophotometric method". Its scope clause reads: This section GB/T 2441 specifies the spectrophotometric determination of biuret in urea content of copper complex salt.
This section applies to the synthesis from ammonia and carbon dioxide obtained biuret urea Determination. Its clauses include reagents and solutions; instruments.
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Document preview — GB/T 2441.2-2010
National Standard of the People's Republic of China
- ICS
- 65.080
- Classification
- G 20
- Replacing
- GB/T 2441.2-2001
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents and solutions
- 5 Instruments
1 Scope
This section GB/T 2441 specifies the spectrophotometric determination of biuret in urea content of copper complex salt.
This section applies to the synthesis from ammonia and carbon dioxide obtained biuret urea Determination.
2 Normative references
The following documents contain provisions which, through reference to GB/T 2441 to the present, constitute provisions of this section. For dated references,
All subsequent amendments (excluding corrections) or revisions do not apply to this section, however, encourage an agreement under this section
Parties to study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
HG/T 2843 fertilizer products for chemical analysis commonly used standard titration solution, standard solution, reagent solution and indicator solution.
Principle 3
Biuret generated purple complex in alkaline solution of copper sulfate, potassium sodium tartrate in, 550nm absorbance was measured at a wavelength of.
4 Reagents and solutions
As used in this section reagent solution and water, in the specifications and method of preparation is not specified, it shall comply with the provisions of HG/T 2843's.
4.1
copper sulfate solution, 15g/L;
4.2
alkaline potassium sodium tartrate solution, 50g/L;
4.3
biuret standard solution, 2.00g/L.
5 Instruments
5.1
usually laboratory instruments;
5.2
a water bath at 30 °C ± 5 °C;
5.3
Spectrophotometer with 3cm absorption cell.
Step 6 Analysis
Do two parallel determination of sample.
Draw standard curve 6.1 6.1.1 Preparation of standard colorimetric solution.
6.1.2
as shown in Table 1, the biuret standard solution were injected sequentially eight 100mL flask.
Table 1 biuret standard solution amount
Biuret standard solution volume/mL biuret corresponding amount/mg
00
GB/T 2441.8-2010 | GB/T 2441.7-2010 | GB 8569-2009 | GB/T 2441.6-2010 | GB/T 2441.5-2010 | GB/T 2441.4-2010 | […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Similar standards
Editions of GB/T 2441.2
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 2441.2-2010 | Determination of urea - Part 2: Biuret content - Spectrophotometric method | current edition | Current |
| GB/T 2441.2-2001 | Determination of urea - Part 2: Biuret content - Spectrophotometric method | previous edition | Superseded |
This page sells the current edition, GB/T 2441.2-2010. Earlier editions are listed for reference only.
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Related Standards
GB/T 2441.1-2008 — Determination of urea - Part 1: Total nitrogen content
GB/T 2441.3-2010 — Determination of urea - Part 3: Water content - Karl Fischer method
GB/T 2441.4-2010 — Determination of urea -- Part 4: Iron content. 1, 10-Phenanthroline spectrophotometric method
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GB/T 2441.2-2010
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