GB/T 23877-2009Determination of citric acid, fumaric acid and lactic acid in feed acidifier - High performance liquid chromatography (English PDF)

1 Scope

This standard specifies the determination of the feed acidifiers citric acid, fumaric acid and lactic acid content by HPLC.

This standard applies to feed acidifiers are citric acid, fumaric acid, lactic acid levels measured.

Quantification limit of the method is. citrate 800mg/kg, fumaric acid 8.0mg/kg, lactic acid 800mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

Preparation of GB/T 601 chemical reagent standard titration solution

Laboratory use specifications and test methods GB/T 6682 Analysis

GB/T 14699.1 feed samples

Principle 3

Sample citric acid, fumaric acid and lactic acid with water after ultrasonic extraction, diluted to the appropriate concentration was measured by high performance liquid chromatography, outside

Calculate the content of citric acid, fumaric acid and lactic standard method.

4 Reagents and materials

Unless otherwise indicated, the methods used were of analytical grade reagents and water to comply with GB/T 6682 Category 2 water.