GB/T 23187-2008Determination of lutein in feeds - High performance liquid chromatography (English PDF)

1 Scope

This standard specifies the method of high performance liquid chromatography in feed lutein.

This standard applies to single feed, feed additive premix, concentrated feed and feed with the determination of lutein. The limit of quantification

Of 0.5mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT)

GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT)

Principle 3

Lutein sample after alkaline saponification and solvent extraction, the supernatant was filtered, high performance liquid chromatography separation with purple

External detector and calculate the content of lutein external standard method.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade reagent water in line with GB/T 6682 stipulated a water.

5 Instruments