GB/T 22916-2008Protocol of fluorogenic RT-PCR for vesicular stomatitis virus (English PDF)

Foreword

The standard reference to the OIE (OIE) "Terrestrial Animals Manual of diagnostic tests and vaccines (mammals, birds and bees)" (5th edition). Appendix A of this standard is a normative appendix, Appendix B is an informative annex. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Animal Epidemic Prevention. This standard was drafted. People's Republic of China SZCIQ, People's Republic of China Yunnan Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. flower Qun Yi, Zhou Xiaoli, Zeng Ling, Caochen Fu, Zhanai Jun, Zhang Caihong, Lin Qingyan, Chen Bing, Yang Yunqing, Sun Jie. Vesicular stomatitis virus fluorescent RT-PCR detection method

1 Scope

GB/T 22916-2008 is the Chinese national standard on protocol of fluorogenic rt-pcr for vesicular stomatitis virus, in the field of health care technology. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS 11.220, CCS B41. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This standard specifies the method of operation of vesicular stomatitis virus fluorescent RT-PCR detection. This standard applies to animals and their products in detecting vesicular stomatitis virus.

2 Acronyms

The following abbreviations apply to this standard.

2.1 fluorescent RT-PCR Fluorescence reverse transcription - polymerase chain reaction. The number of cycles when fluorescence signal of each reaction tube reaches a set threshold experienced.

2.4 DEPC Dicarbonate phospholipids.

2.5 PBS Phosphate buffered saline (recipe see Appendix A).

2.6 T Rao enzyme q T Rao q DNA polymerase.