GB/T 22728-2008Determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in cosmetics - High performance liquid chromatography (English PDF)
化妆品中丁基羟基茴香醚（BHA）和二丁基羟基甲苯（BHT）的测定 高效液相色谱法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
August 1, 2009
Scope
GB/T 22728-2008 is the English-translated version of 化妆品中丁基羟基茴香醚（BHA）和二丁基羟基甲苯（BHT）的测定 高效液相色谱法.
GB/T 22728-2008 is the Chinese standard "Determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in cosmetics - High performance liquid chromatography".
Its scope clause reads: This standard specifies the method for liquid chromatography cosmetics butyl hydroxy anisole (BHA) and butylated hydroxytoluene (BHT) of.
This standard is applicable to solid, pasty and liquid cosmetics butyl hydroxy anisole (BHA) and butylated hydroxytoluene (BHT) of Determination. The standard method detection limit. butylated hydroxy anisole (BHA), butylated hydroxytoluene (BHT) are 0.005%.
Principle 2 Extracted with methanol by ultrasonic cosmetics BHA, BHT, centrifuged, the supernatant was filtered, and the filtrate was equipped with a UV detector efficiency Liquid chromatography detection, external standard. Its clauses include reagents and materials; instrument; determination.
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Document preview — GB/T 22728-2008
National Standard of the People's Republic of China
- ICS
- 71.100.70
- Classification
- Y 42
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3 Reagents and materials
- 4 Instrument
- 5 Determination
1 Scope
This standard specifies the method for liquid chromatography cosmetics butyl hydroxy anisole (BHA) and butylated hydroxytoluene (BHT) of.
This standard is applicable to solid, pasty and liquid cosmetics butyl hydroxy anisole (BHA) and butylated hydroxytoluene (BHT) of
Determination.
The standard method detection limit. butylated hydroxy anisole (BHA), butylated hydroxytoluene (BHT) are 0.005%.
Principle 2
Extracted with methanol by ultrasonic cosmetics BHA, BHT, centrifuged, the supernatant was filtered, and the filtrate was equipped with a UV detector efficiency
Liquid chromatography detection, external standard.
3 Reagents and materials
Unless otherwise indicated, all reagents were of analytical grade and water as secondary deionized or distilled water.
3.1 methanol
Pure class distinctions or chromatography.
3.2 Standard
BHA, purity of not less than 98% (of which 3-BHA, purity of not less than 90%; 2-BHA, purity of not more than 8%); BHT, purity not less
At 99%.
3.3 stock standard solution
BHA's standard stock solution. Weigh accurately BHA standard 0.1g, accurate to 1mg, dissolved with methanol was transferred to a 100mL capacity
Flask, constant volume of methanol, shaking uniform, that was the BHA concentration of standard stock solution 1000µg/mL of.
Standard stock solution of BHT. BHT standard accurately weighed 0.1g, accurate to 1mg, dissolved with methanol was transferred to a 100mL capacity
Flask, constant volume of methanol, shaking uniform, that was BHT concentration of standard stock solution 1000µg/mL of.
4 Instrument
4.1 High performance liquid chromatograph
with UV detector.
4.2
ultrasonic cleaner.
4.3 high-speed centrifuge
not less than 15000r/min.
4.4 syringe filter samples
the organic phase with a membrane, the membrane pore size of 0.45µm.
4.5 conical flask
a grinding gag, 50mL.
4.6 flask
100mL, 25mL.
5 Determination
5.1 Sample Processing
5.1.1 solid, pasty Cosmetics
Cosmetics weighed 0.5g (accurate to 1mg) in 50mL conical flask, methanol was added 25.00mL, super
Sound extraction 20min, to 12000r/min high-speed centrifugal 15min, supernatant was 0.45µm filter, filtrate for testing.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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