GB/T 21814-2008Testing methods for industrial wastewater -- Fish acute toxicity (English PDF)

Foreword

This standard is identical with the Japanese Industrial Standards JISK0102-71:1998 "Test methods of industrial wastewater fish acute toxicity test" (Japanese version).

This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.

This standard was drafted. Chinese Academy of Inspection and Quarantine, the Ministry of Environment Protection Standards Institute, Liaoning CIQ.

1 Scope

This standard specifies the laboratory determination of industrial waste water fish acute toxicity test scope, methodology overview, test preparation, test equipment, specimen the collection and storage, test conditions, test procedure and test results.

This standard applies to the determination of the acute toxicity of industrial waste water fish.

2 Methods Summary

By this standard fish median lethal concentration LC50 (Medianlethalconcentration) assess fish acute toxicity test substance.

Feeding trials some time in an aqueous solution of the test substance in fish to 96h as a test cycle, 24h, 48h, 72h and 96h when Hutchison Fish mortality recorded test to determine the concentration of test substance in fish deaths by 50%, with a median lethal concentration 24hLC50,48hLC50, 72hLC50 and 96hLC50 representation.

NOTE. LC50 fish is not an acceptable concentration of no effect, no effect of fish acceptable concentration required by the subacute toxicity, chronic toxicity test was measured. LC50 Value multiplied by an appropriate coefficient can be estimated fish acceptable concentration had no effect, as is usually 48hLC50 multiplication factor of about 0.1, 96hLC50 Multiplication factor of about 0.01. Test fish species, health status and dilution water quality, water temperature and so will have an impact LC50, and and the impact of this quantitative relationship is unclear, so when the waters of the biological effects of emissions on the study can be selected with the discharge waters natural conditions Similar pieces of fish and water dilution test. When comparing different substances toxicity or toxicity studies the causes, the same should be used for the test species and test Experimental conditions. This standard describes the determination of the LC50, the test conditions as close as possible without departing from the discharge waters this purpose.

3 Test preparation