GB/T 21321-2007Method for determination of avermectins residue in animal origin food - Immunoaffinity -high performance liquid chromatograph (English PDF)

Foreword

Appendix A of this standard is an informative annex.

1 Scope

This standard specifies the food of animal origin avermectin residues of drugs detected by immune affinity - liquid chromatography.

This standard applies to bovine liver and bovine muscle tissue samples avermectin, ivermectin, doramectin and Eprinomectin Residues Determination.

The standard method detection limit. abamectin, ivermectin, doramectin and Eprinomectin were 1µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Principle

The sample was extracted with methanol of four avermectin by immunoaffinity column, eluted with methanol. Eluent blowing with nitrogen Dry, after derivatization by high performance liquid chromatography - fluorescence detection, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.

4.1 Methanol. chromatographically pure.

4.2 Acetonitrile. chromatography.

4.3 Potassium dihydrogen phosphate.

4.4 Potassium chloride.

4.5 Disodium hydrogen phosphate (Na2HPO4 . 12H2O).

4.6 Sodium chloride.

4.7 of acetic anhydride.

4.8 1-N- methylimidazole. content of >=99%.

4.9 Eprinomectin standard. Purity >=98%.

4.10 Avermectin standard. Purity >=98%.

4.11 Doramectin standards. purity >=94.3%.

Ivermectin 4.12 Standard. Purity >=98%.

4.13 Immunoaffinity column (IAC column). length 4cm, the inner diameter of 1cm, IAC gel volume of 1mL.

4.14 Organization dilution. 0.01mol/L phosphate buffer (pH7.4). Weigh potassium dihydrogen phosphate (4.3) 0.2g, potassium chloride (4.4) 0.2g, disodium hydrogen phosphate (4.5) 2.9g, Sodium Chloride (4.6) 8.8g, dissolved in water and diluted to 1L.

4.15 washing solution. A solution, B solution and C solution. A solution of 0.01mol/L phosphate buffer (4.14) + methanol (80 + 20, by volume); Solution B was 20% methanol solution; the C solution of 50% methanol solution.