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GB/T 44742-2024Determination of sea cucumber polysaccharides in sea cucumber and its products—High performance liquid chromatography (English PDF)

海参及其制品中海参多糖的测定高效液相色谱法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

October 26, 2024

Implementation date

May 1, 2025

Scope

GB/T 44742-2024 is the English-translated version of 海参及其制品中海参多糖的测定高效液相色谱法.

GB/T 44742-2024 is the Chinese national standard covering the sea cucumber polysaccharide in sea cucumber and in sea cucumber powder — the enzymatic digestion with papain, the precipitation with potassium acetate and the acid hydrolysis that free the sugar, the derivatisation of the released fucose, the separation on a C18 column with ultraviolet detection, and the calculation that reads the polysaccharide content from the fucose alone, so the figure rests on a marker sugar rather than on total carbohydrate. Issued the same day as the wolfberry polysaccharide method GB/T 44739-2024. First edition, in force from 1 May 2025. Issued on 26 October 2024, it has been in force since 1 May 2025.

Document preview — GB/T 44742-2024

National Standard of the People's Republic of China

ICS: 67.120
Classification: B 51

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Foreword
1 Scope
2 Normative references
3 Terms and Definitions
3.1
4 Principles
5 Reagents and Materials
5.1 Reagents

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China National Institute of Standardization.

This document was drafted by: Ocean University of China, China National Institute of Standardization, Shandong Haodangjia Ocean Development Co., Ltd., Dalian Xiaoqin Food Co., Ltd., Qingdao Marine Food Nutrition and Health Innovation Research Institute, Quanzhou Marine Biological Industry Research Institute, SGS-CSTC Standards Technical Services Co., Ltd.

Co., Ltd. Dalian Branch, Zhongshan Hongli Healthy Food Industry Research Institute Co., Ltd.

The main drafters of this document are: Xue Changhu, Fan Yan, Wang Yuming, Mao Xiangchao, Hou Hu, Xi Xingjun, Hu Wei, Liang Weigang, Sun Yongjun, Li Chao, He Chunli, Feng Tingyu, Li Ruoshu, Liu Shoufeng, Luan Wenwen, and Lei Minzhi.

Determination of sea cucumber polysaccharides in sea cucumber and its products High-performance liquid chromatography

1 Scope

This document describes a method for the determination of sea cucumber polysaccharides in sea cucumbers and their products using high performance liquid chromatography.

This document is applicable to the determination of sea cucumber polysaccharides in sea cucumbers and sea cucumber powder.

2 Normative references

GB/T 6682

3 Terms and Definitions

The following terms and definitions apply to this document.

3.1

A type of polysaccharide composed of sea cucumber fucoidan sulfate and sea cucumber chondroitin sulfate.

Note. Sea cucumber fucoidan sulfate is a linear sulfated polysaccharide formed by the polymerization of L-fucose; sea cucumber chondroitin sulfate is a type of polysaccharide with fucose side chains.

The substituted sulfated polysaccharide is mainly composed of D-acetylgalactosamine, D-glucuronic acid and L-fucose.

4 Principles

The sample was enzymatically hydrolyzed, potassium acetate precipitated, and acid hydrolyzed to obtain sea cucumber polysaccharide hydrolyzate. The fucose in the hydrolyzate was mixed with 1-phenyl-3-methyl-5-pyridinium chloride.

The product was separated by C18 column and determined by high performance liquid chromatography equipped with UV detector.

The content of sea cucumber polysaccharides in the sample was identified by the fucose content.

5 Reagents and Materials

5.1 Reagents

5.1.1 Water. GB/T 6682, Grade 1.

5.1.2 Papain. Food grade, enzyme activity >= 100,000 U/g.

5.1.3 Ethylenediaminetetraacetic acid (C10H16N2O8).

5.1.4 Cysteine hydrochloride (H-Cys-OHHCl).

5.1.5 Potassium acetate (CH3COOK). 5.1.6 Chloroform (CHCl3).

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 6682Water for analytical laboratory use - Specification and test methods

Editions of GB/T 44742

Edition	Title	Revision	Status
GB/T 44742-2024	[Including 2026XG1] Determination of sea cucumber polysaccharides in sea cucumber and its products - High performance liquid chromatography	current edition	Current

This page sells the current edition, GB/T 44742-2024. Earlier editions are listed for reference only.

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