GB/T 21318-2007Determination of nitroimidazoles residues in foodstuffs of animal origin (English PDF)

1 Scope

This standard specifies the foods of animal origin nitroimidazole original eight kinds of drugs, metabolites two kinds of residues Performance Liquid Chromatography/Tandem Mass Spectrometry test methods.

This standard applies to pork, chicken, beef, liver, chicken liver, beef liver, kidney, beef kidney, fish, milk, honey. 4-nitroimidazole, isopropyl

Metronidazole, 2-methyl imidazole nitrate, Ronidazole, metronidazole, metronidazole imidazole chlorine, Dimetridazole, benzene imidazole nitrate residues of metronidazole and its metabolites hydroxy

MNZOH (metronidazole metabolite), hydroxymethyl metronidazole imidazole HMMNI (2- methyl imidazole nitrate, Ronidazole metabolite) were measured.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

Principle 3

Was performed on eight-nitroimidazole, two kinds of metabolites with methanol - acetone homogeneous or ultrasonic extraction, liquid-liquid partition with ethyl acetate, to

Gel Permeation Chromatography (GPC) purification, and then by solid phase extraction (SPE) purification by liquid chromatography/tandem mass spectrometry confirmed, external standard quantitative measurement.

4 Reagents and materials

Unless otherwise noted, all reagents were of analytical grade water as a water GB/T 6682 regulations.