GB/T 21317-2007Determination of tetracyclines residues in food of animal origin - LC-MS/MS method and HPLC method (English PDF)

1 Scope

This standard specifies the animal derived food preparation methods Tetracycline Residues in detection, HPLC method and the liquid

GC - MS/MS confirmation methods.

This standard applies to animal muscle, visceral, fish, milk and other foods of animal origin minocycline, oxytetracycline, tetracycline,

HPLC norepinephrine fund, chlortetracycline, methylene oxytetracycline, doxycycline 7 kinds of tetracyclines veterinary drug residues and dimethylamine

Tetracycline, oxytetracycline to the poor, oxytetracycline, to the poor, tetracycline, Demeclocycline difference to chlortetracycline, chlortetracycline, oxytetracycline A strong

Doxycycline 10 kinds of tetracyclines residues in liquid chromatography - mass spectrometry/mass spectrometry.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

Principle 3

Tetracycline Antibiotic Residues in the sample was extracted with 0.1mol/LNa2EDTA-Mclvaine buffer (pH = 4.0 ± 0.05), by

After filtration and centrifugation, the supernatant with HLB solid-phase extraction (SPE), high performance liquid chromatography or liquid chromatography electrospray ionization mass spectrometer, external standard

Peak area quantification.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.