GB/T 21100-2007Identification of camelidae derived material in animal - originated feedstuff PCR method (English PDF)
动物源性饲料中骆驼源性成分定性检测方法 PCR方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
October 24, 2007
Implementation date
April 1, 2008
Scope
GB/T 21100-2007 is the English-translated version of 动物源性饲料中骆驼源性成分定性检测方法 PCR方法.
GB/T 21100-2007 is the Chinese standard "Identification of camelidae derived material in animal - originated feedstuff PCR method". Its scope clause reads: This standard specifies the PCR method of animal feed camels (Camelidae) derived materials testing, the detection limit of the assay 0.1% (mass fraction).
This standard applies to animal feed in the qualitative detection camelized ingredient. Its clauses include terms and definitions; reagents and materials.
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Document preview — GB/T 21100-2007
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 5 Reagents and materials
1 Scope
This standard specifies the PCR method of animal feed camels (Camelidae) derived materials testing, the detection limit of the assay
0.1% (mass fraction).
This standard applies to animal feed in the qualitative detection camelized ingredient.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB 6682 Analysis
GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT)
SN/T 1193 genetic testing laboratory technical requirements
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Located two known for amplification of DNA (deoxyribonucleosideaacid, DNA) sequences of the method. template
High temperature DNA denaturation into single strands, DNA polymerase and at a suitable temperature, two mutually complementary oligonucleotide primers fragment that is divided
Do annealing occurs with some complementary sequence on both strands of the template DNA, followed by catalytic DNA polymerase in four dNTP (de- oxyribonucleosidetriphosphate, deoxynucleotide triphosphate) as substrate, annealing the primers to be extended, so repeated denaturation, annealing
DNA synthesis and the circulation, located in two DNA fragments of known sequences were amplified geometrically, by 25 to 30 amplification
Cycle, the expansion fold to about 106.
Principle 4
Use of cell lysate broken, chloroform extraction of proteins, ethanol precipitation to give DNA; with the extracted DNA as a template
PCR amplification and agarose gel electrophoresis to detect PCR amplification products; endo-positive PCR product was digested with restriction enzyme reactions were confirmed.
5 Reagents and materials
Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water line with GB 6682 requirements.
5.1
Identification of Camelidae component detection primer (pair) sequence.
F. 5'-AGCCTTCTCTTCAGTCGCACAC-3 '
R. 5'-GCCCATGAAAGCTGTTGCT-3 '
5.3 endo restriction enzymes
T Rao q I enzyme.
5.4 dNTPs
dATP (deoxyadenosinetriphosphate, deoxyadenosine triphosphate), dTTP (deoxythymidinetriphos- […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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