GB/T 20806-2022Determination of neutral detergent fiber (NDF) in feeds (English PDF)
饲料中中性洗涤纤维（NDF）的测定
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 30, 2022
Implementation date
July 1, 2023
Scope
GB/T 20806-2022 is the English-translated version of 饲料中中性洗涤纤维（NDF）的测定.
GB/T 20806-2022 describes how to measure neutral detergent fibre (NDF) in feed, by the crucible method, which is the arbitration method, and by the filter bag method.
The sample is digested and washed with thermostable alpha-amylase and neutral detergent solution to remove the easily digestible protein, lipid, sugar, starch and pectin; what remains is the insoluble fibre residue, mainly cellulose, hemicellulose and lignin.
The document lists the reagents and how to prepare them, among them grade III water to GB/T 6682, anhydrous sodium sulfite, acetone, 4 mol/L hydrochloric acid and the neutral detergent solution adjusted to pH 6.95 to 7.05, and its annex covers calibrating and diluting the amylase.
It applies to compound feed, concentrated feed, concentrate supplement and feed raw materials. It replaces GB/T 20806-2006, was issued on 30 December 2022 and has applied since 1 July 2023.
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Document preview — GB/T 20806-2022
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
- Replacing
- GB/T 20806-2006
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 4 Crucible method (arbitration method)
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.
This document replaces GB/T 20806-2006 "Determination of Neutral Detergent Fiber (NDF) in Feed", and is consistent with GB/T 20806-2006 In addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) Change the sample fineness requirements (see 4.4, 7.2 of the 2006 edition);
b) Added a pre-degreasing step (see 4.5.3);
c) Added "0.5g anhydrous sodium sulfite" for digestion (see 4.5.4);
d) Increased filter aid (see 4.5.5);
e) Changed the test data processing (see 4.6, 8.1 of the 2006 edition);
f) The filter bag method is added (see Chapter 5);
g) Increased calibration and dilution of thermostable alpha-amylase (see Appendix A).
1 Scope
This document describes the crucible and filter bag methods for the determination of neutral detergent fiber (NDF) in feed.
This document is applicable to the determination of neutral detergent fiber (NDF) in compound feed, concentrated feed, concentrate supplement and feed raw materials.
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 20195 Preparation of Animal Feed Samples
3 Terms and Definitions
This document does not have terms and definitions that need to be defined.
4 Crucible method (arbitration method)
4.1 Principle
Digest and wash with thermostable alpha-amylase and neutral washing solution to remove easily digestible protein, lipid, sugar, starch and fruit in the sample.
After gluing, what remains is insoluble fiber residues, mainly vegetable raw material components such as cellulose, hemicellulose and lignin, and animal products. Indigestible nitrogenous substances.
4.2 Reagents or materials
Unless otherwise specified, use only analytical grade reagents.
4.2.1 Water: GB/T 6682, Class III.
4.2.2 Anhydrous sodium sulfite (Na2SO3).
4.2.3 Acetone.
4.2.4 Hydrochloric acid solution (4mol/L): Measure 328mL of concentrated hydrochloric acid, dilute with water to 1000mL, and mix well.
4.2.5 Thermostable alpha-amylase solution: purchase thermostable alpha-amylase. Calibrate and test thermostable alpha-amylase according to Appendix A before use Dilute to ensure that the diluted 2mL thermostable alpha-amylase solution can remove the starch in 0.5g raw corn flour. Diluted heat stable alpha- The amylase solution is stored at 2°C~8°C, and the validity period is 5 days.
4.2.6 Neutral washing solution: Measure 500mL of water into a 1000mL beaker, add 18.6g of disodium edetate (C10H14 N2O8Na2 2H2O), 4.56g anhydrous disodium hydrogen phosphate (Na2HPO4) and 6.81g sodium tetraborate (Na2B4O7 10H2O), heating Dissolve, mix well, and place in a fume hood. Add 30g sodium dodecyl sulfate (C12H25NaO4S), dissolve and add 10mL triethylene diethylene disulfide Alcohol (C6H14O4) to eliminate foam, add water to about 950mL, stir evenly, use concentrated hydrochloric acid or sodium hydroxide to adjust the pH to 6.95~7.05, water Dilute to 1000mL. Store at room temperature.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Similar standards
Editions of GB/T 20806
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 20806-2022 | Determination of neutral detergent fiber (NDF) in feeds | current edition | Current |
| GB/T 20806-2006 | Determination of neutral detergent fiber (NDF) in feeds | previous edition | Superseded |
This page sells the current edition, GB/T 20806-2022. Earlier editions are listed for reference only.
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