GB/T 19423-2020Determination of nicarbazin in feeds (English PDF)
饲料中尼卡巴嗪的测定
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 14, 2020
Implementation date
July 1, 2021
Scope
GB/T 19423-2020 is the English-translated version of 饲料中尼卡巴嗪的测定.
GB/T 19423-2020 sets the determination of nicarbazin in feeds. It gives two methods, high performance liquid chromatography and liquid chromatography-tandem mass spectrometry, with their reagents, apparatus, procedure and calculation.
It applies to compound feeds, concentrated feeds and additive premixes. Feed makers, feed control laboratories and regulators use it. Issued on 14 December 2020, it has applied since 1 July 2021, replacing GB/T 19423-2003.
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Document preview — GB/T 19423-2020
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
- Replacing
- GB/T 19423-2003
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 High performance liquid chromatography
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 Standardization Guidelines Part 1: Structure and Drafting Rules of Standardization Documents Drafting.
This document replaces GB/T 19423-2003 "Determination of Nicarbazine in Feed High-Performance Liquid Chromatography", and GB/T 19423- Compared with 2003, in addition to editorial changes, the main technical changes are as follows:
---Added liquid chromatography-tandem mass spectrometry method (see Chapter 5);
---Changed the detection limit of high performance liquid chromatography and increased the limit of quantification (see Chapter 1, Chapter 1 of the 2003 edition);
---Changed the extraction and purification methods of high performance liquid chromatography (see 4.5.1, 4.5.2, 7.1 in the 2003 edition);
---Changed the test data processing of high performance liquid chromatography (see 4.6, 7.4 in the 2003 edition).
Please note that certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
1 Scope
This document describes the high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry methods for the determination of Nicarbazine in feed.
This document is applicable to the determination of Nicarbazine in compound feed, concentrated feed and additive premix feed.
The detection limit of compound feed and concentrated feed in this document is 0.5mg/kg, and the limit of quantification is 1mg/kg. Additives The detection limit of premixed feed is 1 mg/kg, and the limit of quantification is 2 mg/kg; the detection limit of liquid chromatography-tandem mass spectrometry is 0.02 mg/kg, The limit is 0.05mg/kg.
2 Normative references
The contents of the following documents constitute indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 High performance liquid chromatography
4.1 Principle
Nicarbazine in the sample is extracted with a methanol-acetonitrile mixed solution, purified by MAX solid phase extraction cartridge, and then used by high performance liquid chromatography Detect and quantify by external standard method.
4.2 Reagents or materials
Unless otherwise specified, only use analytical reagents.
4.2.1 Water. GB/T 6682, level one.
4.2.2 Acetonitrile. chromatographically pure.
4.2.3 Extraction solution. methanol acetonitrile = 50 50.
4.2.4 80% acetonitrile solution. accurately measure 80mL acetonitrile (4.2.2), add water to the volume to 100mL, and mix.
4.2.5 Sodium hydroxide solution (0.2mol/L). accurately measure 0.8g of sodium hydroxide, dilute with water and dilute to 100mL, and mix.
4.2.6 4% formic acid acetonitrile solution. accurately measure 4mL formic acid, dilute with acetonitrile (4.2.2) and dilute to 100mL, and mix.
4.2.7 Standard stock solution (1000µg/mL). Weigh Nicarbazin (Nicarbazin, C19H18N6O6, CAS number. 330-95-0, content Not less than 99%) standard 10mg (accurate to 0.01mg) in a 10mL volumetric flask, add 2mL dimethyl sulfoxide to dissolve, use acetonitrile (4.2.2) Constant volume, shake well, store below -18°C, validity period is 3 months.
4.2.8 Standard intermediate solution (100µg/mL). accurately measure 1mL of the standard stock solution (4.2.7) and place it in a 10mL volumetric flask. […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Similar standards
Editions of GB/T 19423
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 19423-2020 | Determination of nicarbazin in feeds | current edition | Current |
| GB/T 19423-2003 | Determination of nicarbazin in feeds | previous edition | Superseded |
This page sells the current edition, GB/T 19423-2020. Earlier editions are listed for reference only.
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Related Standards
GB/T 20195-2006 — Animal feeding stuffs - Preparation of test samples
GB/T 20195-2024 — Animal feed - Preparation of test samples
GB/T 6682-1992 — Water for analytical laboratory use--Specification and test methods
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