GB/T 18086-2000Plant quarantine--Methods for inspection and identification of Peronospora hyoscyami de Bary f.sp.tabacina(Adam)Skalichy (English PDF)
植物检疫 烟霜霉病菌检疫鉴定方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
April 26, 2000
Implementation date
October 1, 2000
Scope
GB/T 18086-2000 is the English-translated version of 植物检疫 烟霜霉病菌检疫鉴定方法.
GB/T 18086-2000 is the Chinese standard "Plant quarantine--Methods for inspection and identification of Peronospora hyoscyami de Bary f.sp.tabacina(Adam)Skalichy".
Its scope clause reads: This standard specifies the method for inspection and identification of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy in import plant quarantine.
This standard is applicable to inspection and identification of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy in import dry tobacco leaves and seeds and seedlings of nicotiana plants. Its clauses include principle; instruments and reagents.
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Document preview — GB/T 18086-2000
National Standard of the People's Republic of China
- ICS
- 65.020
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword i
- 1 Scope
- 2 Principle
- 3 Instruments and reagents
- 4 Sampling
- 5 Inspection methods of tobacco leaves
- 6 Inspection methods for seeds and seedlings of nicotiana plant
- 7 Identification characteristics
- 8 Result judgment
- Annex A (Informative) Oospore inspection by frozen homogenization method
1 Scope
This standard specifies the method for inspection and identification of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy in import plant quarantine.
This standard is applicable to inspection and identification of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy in import dry tobacco leaves and seeds and seedlings of nicotiana plants.
2 Principle
Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy is an obligate parasite. After tobacco leaves are infected, nearly round spots with blurred edges and chlorosis will appear, and later pale brown necrotic spots that can fall off will form. Under suitable temperature and humidity conditions, light blue mold [namely, the sporangiophore and sporangium of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy] will be formed on the necrotic spot on the back of leaves; under certain conditions, oospore may be formed in diseased tissues. Parasitic specialization and morphological characteristics of pathogenic bacteria are the basis for the identification of Peronospora hyoscyami de Bary f. sp. tabacina (Adam) Skalichy.
3 Instruments and reagents
3.2
Magnifier with light source.
3.3
Binocular dissecting microscope (10×~50×).
3.4
Microscope (100×~1 000×).
3.5
Centrifuge (1 000r/min ~ 4 000r/min).
3.6
Stainless steel metal screen (aperture size of 60µm).
3.7
Oscillator (200r/min).
3.8
Phosphate buffer solution, 0.02mol/L (pH=7).
3.10
Phenol glycerin solution.
Preparation of phenol glycerin solution: mixture of 20mL of phenol crystal (dissolved by heating in water bath), 20mL of lactic acid, 40mL of glycerinum and 20mL of distilled water.
Preparation of potassium hydroxide solution: taking 10g of potassium hydroxide and dissolving with 90mL of water to form 10% potassium hydroxide solution.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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