GB/T 17815-2018Determination of propionic acid, propionate in feeds (English PDF)
饲料中丙酸、丙酸盐的测定
This is a limited preview
Buy now to download the full PDF (15 pages)
Need quotation for your management?
Get a formal quotation for GB/T 17815-2018 in 30 minutes.
Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
September 17, 2018
Implementation date
April 1, 2019
Scope
GB/T 17815-2018 is the English-translated version of 饲料中丙酸、丙酸盐的测定.
GB/T 17815-2018 sets out how to determine propionic acid and propionates in feeds, with gas chromatography as the arbitration method and high performance liquid chromatography as the second method.
It applies to feed materials, compound feeds and concentrated feeds whose propionic acid content, expressed as propionic acid, is below 3000 mg/kg. For both methods the detection limit is 30 mg/kg and the limit of quantitation 100 mg/kg.
In the gas chromatographic method the sample is acidified with phosphoric acid, propionic acid is separated by steam distillation and measured with a flame ionization detector against an external standard.
Compared with the 1999 edition it uses a capillary column, reports results in mg/kg and adds the liquid chromatographic method. It replaces GB/T 17815-1999, was issued on 17 September 2018 and has applied since 1 April 2019.
Found what you need?
Document preview — GB/T 17815-2018
National Standard of the People's Republic of China
- ICS
- 65.120
- Classification
- B 46
- Replacing
- GB/T 17815-1999
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Gas chromatography (arbitration method)
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 17815-1999 "Determination of propionic acid and propionate in feed".
This standard is compared with GB/T 17815-1999 "Determination of propionic acid and propionate in feed", except for editorial modification, the main technical content The changes are as follows.
--- Increase the detection limit and limit of quantitation of the method (see Chapter 1);
--- Revised the concentration of phosphoric acid in the preparation of sample solution (see 3.5.1, 1999 edition 7.1);
--- Increased reagent quartz sand and formic acid in the preparation of sample solution (see 3.5.1);
--- The gas chromatographic column was changed from packed column to capillary column, and the chromatographic conditions were changed accordingly. The quantification was changed from the internal standard method to the external standard method (see 3.5.3, 1999 edition 7.2);
--- Increased liquid chromatography;
--- The result unit is changed from "%" to "mg/kg" (see 3.6, Chapter 8 of the 1999 edition);
--- Added informative appendix.
1 Scope
This standard specifies the gas chromatographic and high performance liquid chromatography methods for the determination of propionic acid and propionate in feed.
This standard is applicable to feed materials, compound feeds, concentrated feeds of propionic acid and propionate with propionic acid content below 3000mg/kg (to C Determination of acid meter).
The detection limit of gas chromatography in this standard is 30mg/kg, the limit of quantification is 100mg/kg; the detection limit of high performance liquid chromatography is 30mg/kg, the limit of quantification is 100mg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14699.1 Feed sampling
Preparation of GB/T 20195 animal feed samples
3 Gas chromatography (arbitration method)
3.1 Principle
The propionic acid and propionate in the sample are acidified by phosphoric acid, the propionate is converted into propionic acid, and the steam distillation separates the propionic acid from the substrate, and the distillate is separated.
Propionic acid was detected by gas chromatograph with hydrogen flame ionization detector and quantified by external standard method.
3.2 Reagents or materials
Only analytically pure reagents were used in the analysis unless otherwise stated.
3.2.1 Water, GB/T 6682, level one.
3.2.2 Phosphoric acid. excellent grade pure.
3.2.3 Sodium chloride.
3.2.4 Quartz sand.
3.2.5 Silicone oil.
3.2.6 Phosphoric acid solution (1 1). Dilute 50 mL of phosphoric acid to 100 mL with water and mix.
3.2.7 Formic acid solution (2 98). Pipette 2 mL of formic acid to 100 mL with water and mix.
3.2.8 Propionic acid standard stock solution. Accurately weigh 1.0310g propionic acid (C3H6O2, purity >=97%) in a 100mL volumetric flask, add water Dissolve and bring up to volume. Shake well. 1 mL of this solution contained 10 mg of propionic acid, and this solution was stored at 4 ° C for 30 days.
3.2.9 Propionic acid standard working solution. Pipette the standard stock solution of propionic acid (3.2.8) 10.0mL into a 100mL volumetric flask and dilute with water. Rong. 1 mL of this solution contains 1 mg of propionic acid, which is ready for use.
3.2.10 0.45 µm filter.
3.3 Instrumentation
3.3.1 Gas Chromatograph. with a hydrogen flame ionization detector. […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Similar standards
Editions of GB/T 17815
| Edition | Title | Revision | Status |
|---|---|---|---|
| GB/T 17815-2018 | Determination of propionic acid, propionate in feeds | current edition | Current |
| GB/T 17815-1999 | Determination of propionic acid, propionate in feeds | previous edition | Superseded |
This page sells the current edition, GB/T 17815-2018. Earlier editions are listed for reference only.
How to Download GB/T 17815-2018
- 1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
- 2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
- 3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
- 4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.
Related Standards
GB/T 14699.1-1993 — Method for sampling of feed
GB/T 14699.1-2005 — Feeding stuffs -- Sampling
GB/T 20195-2006 — Animal feeding stuffs - Preparation of test samples
Secure payment via Stripe
Payments accepted
GB/T 17815-2018
$145.00