GB/T 17507-2008General specification of thin biological standards for X-ray EDS microanalysis in transmission electron microscope (English PDF)

2.1

Means cut using a microtome to a thickness of 50nm ~ 300nm in the biological sample.

2.2

Biological samples and biological standards of chemical composition of elements factor. The average atomic number G Available formula (1) is calculated.

G = 

Where.

3.1

Physical properties of thin biological standards for materials, the chemical properties to be consistent with the biological sample to be analyzed thin as possible. Thin biological standards for the

Element concentration recommended by atomic absorption spectrometry, and gives the element's value bias.

3.2

for the preparation of thin biological standards for a matrix material having an average atomic number to be close to the average atomic number of biological samples, was 3.28.

3.3

thin biological standards for should have a high resistance to electron radiation and contamination resistance.

Parent should have sufficient amount of 3.4 standard materials, in addition to setting a sufficient supply of chemical consumption, after being confirmed guarantee made more than 200

Standard products.

3.5

thin biological standards for determining the uniformity index should be less than 3.

3.6

thin biological standards for determining the stability of the index should be less than 3.

3.7

thin biological standards for the concentration of the element error shall be less than ± 5%.

3.8

The thickness of thin biological standards should be within the range of 50nm ~ 300nm.

3.9

Preparation of thin biological standards for should be equal to a diameter of less than 3mm thin slices, available single-hole or porous nylon mesh support, a carbon support network

And other low background material support network gripping.

4.1 initial inspection standards

4.1.1

observed with an optical microscope, thin biological standards for primaries a uniform, smooth, clean and undamaged standard.