GB/T 14643.4-2009Examination of bacteria and algae in industrial circulating cooling water - Part 4: Examination of soil fangi - Standard of plate count (English PDF)

Foreword

GB/T 14643 "Determination of industrial circulating cooling water bacteria and algae" is divided into the following sections.

--- Part 1. Determination of slime-forming bacteria plate count

--- Part 2. Determination of soil bacteria plate count

--- Part 3. slime fungi plate count

--- Part 4. Soil fungi plate count

--- Part 5. Determination of sulfate-reducing bacteria MPN method

--- Part 6. Determination of iron bacteria MPN method

This section GB/T Part of 414,643.

This Part replaces GB/T 14643.4-1993 "soil fungi for industrial circulating cooling water of plate count."

This portion of the GB/T 14643.4-1993 compared to no change in technical content, but the text structure and text of the modify.

1 Scope

GB/T 14643 provisions of this part of the determination of soil fungi for industrial circulating cooling water.

Applies to soil fungi Industrial circulating cooling water - Determination of this section also applies to the raw water, domestic water, and other water and soil fungi Determination.

2 Normative references

The following documents contain provisions which, through GB/T 14643 reference in this text, constitute provisions of this part. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.

GB/T 603 chemical reagent test methods in the formulation and preparation of products (GB/T 603-2002, ISO 6353-1 with. 1982, NEQ)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

This part of soil fungi medium with plate count technique, (29 ± 1) °C culture 72h, Industrial circulating cooling water and soil The total number of soil fungi.

4 Reagents and materials

The reagents used in this section, unless otherwise specified, shall use analytical reagent and in line with GB/T 6682 stipulated three of water.

Test preparation and the desired products, did not indicate when the other requirements, according to GB/T 603 provisions of the preparation.

4.1 Potato. market fresh (no buds), peeled and cut into approximately 20mm × 20mm × 20mm pieces.

4.2 Glucose.

4.3 Agar. biological reagents.

4.4 Sodium chloride.

4.5 Lactic acid.

4.6 Ethanol. 75% (volume fraction).

4.7 Sodium thiosulfate.

4.8 Kraft paper.

4.9 Absorbent.

4.10 Medical gauze.

5 Instruments and equipment

5.1 sterile container (chamber) or clean bench.

5.2 Steam pressure sterilizer.

5.3 Incubator.