GB 31610.6-2023National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products (English PDF)

Also coversGB31610.6-2023

Open the GB 31610.6-2023 preview as PDF

Preview — first pages of GB 31610.6-2023

This is a limited preview

Buy now to download the full PDF

Issued by

National Health Commission of the People's Republic of China, State Administration for Market Regulation

Level / Type

National · Mandatory

Issue date

September 6, 2023

Implementation date

March 6, 2024

Scope

GB 31610.6-2023 (National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products) is available as an English-translated PDF.

GB 31610.6-2023 — This standard specifies the morphology and morphology of Spirometramansoni larvae in animal aquatic products and their products. PCR test method. This standard is applicable to the inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products.

Document preview — GB 31610.6-2023

National Standard of the People's Republic of China

Classification: X 09

Issued by: National Health Commission of the People's Republic of China, State Administration for Market Regulation

1 Scope
2 Principles
3 Instruments and equipment
3.1 Biological microscope. 40x~100x.
3.2 Stereo microscope. 7.5x~150x.
3.3 PCR amplification instrument.
3.4 Gel imaging system.
3.5 Electrophoresis apparatus.
3.6 High-speed centrifuge. speed >=12000r/min.
3.7 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.
4 Reagents and materials
4.1 Reagents

1 Scope

This standard specifies the morphology and morphology of Spirometramansoni larvae in animal aquatic products and their products.

PCR test method.

This standard is applicable to the inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products.

2 Principles

Sparganoid larvae of the tapeworm Mansoni in animal aquatic products and their products mainly live in the muscles of frogs and other aquatic products. Obtained from anatomical separation Based on the morphological structure of the sparganoides, the species of the sparganoides can be preliminarily determined; by amplifying the cells of the mitochondrial DNA of the sparganoid tapeworm Mansoni The cytochrome oxidase (coxI) gene fragment was sequenced to identify the sparganoid larvae of the tapeworm S. mansoni.

3 Instruments and equipment

3.1 Biological microscope. 40x~100x.

3.2 Stereo microscope. 7.5x~150x.

3.3 PCR amplification instrument.

3.4 Gel imaging system.

3.5 Electrophoresis apparatus.

3.6 High-speed centrifuge. speed >=12000r/min.

3.7 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.

4 Reagents and materials

4.1 Reagents

4.1.1 1mol/LTris-HCl solution (pH8.0).

4.1.2 0.5mol/LEDTA solution (pH8.0).

4.1.3 10% SDS solution.

4.1.4 5mol/LNaCl solution.

4.1.5 20mg/mL proteinase K.

4.1.6 Phenol/chloroform/isoamyl alcohol (25.24.1).

4.1.7 Chloroform.

4.1.8 5U/uL thermostable DNA polymerase.

4.1.9 10xPCR buffer.

4.1.10 25mmol/LMgCl2.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — all pages — is available in the English PDF.

How to Buy GB 31610.6-2023

1Add to cart. Click the "Buy GB 31610.6-2023" button on this page. You can add more standards before checkout.
2Checkout. Enter your email and billing details. Payment is processed securely by Stripe (cards, Apple Pay, Google Pay supported).
3Instant delivery (0–9 sec). Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4Invoice included. A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.