GB 31610.5-2023National food safety standards Inspection of Paragonimus in animal aquatic products and their products (English PDF)

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Issued by

National Health Commission of the People's Republic of China, State Administration for Market Regulation

Level / Type

National · Mandatory

Issue date

September 6, 2023

Implementation date

March 6, 2024

Scope

GB 31610.5-2023 (National food safety standards Inspection of Paragonimus in animal aquatic products and their products) is available as an English-translated PDF.

GB 31610.5-2023 — This standard specifies the morphology and PCR testing of Paragonimus metacercariae in animal aquatic products and their products. method. This standard applies to the inspection of Paragonimus metacercariae in animal aquatic products and their products.

Document preview — GB 31610.5-2023

National Standard of the People's Republic of China

Classification: X 09

Issued by: National Health Commission of the People's Republic of China, State Administration for Market Regulation

1 Scope
2 Principles
3 Instruments and equipment
3.1 Biological microscope. 100x~400x.
3.2 Stereo microscope. 7.5x~150x.
3.3 PCR amplification instrument.
3.4 Gel imaging system.
3.5 Electrophoresis apparatus.
3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.
3.7 High-speed centrifuge. speed >=12000r/min.
3.8 Mesh screen. aperture 2mm (10 mesh); aperture 0.425mm (40 mesh).
3.9 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.
3.10 Conical measuring cup. 1000mL.
3.11 Glass beads. 425um~600um.
3.12 Ceramic mortar (diameter 16cm) and grinding rod.
3.13 Cyclotron oscillator.
4 Reagents and materials
4.1 Reagents

1 Scope

This standard specifies the morphology and PCR testing of Paragonimus metacercariae in animal aquatic products and their products.

method.

This standard applies to the inspection of Paragonimus metacercariae in animal aquatic products and their products.

2 Principles

Metacercariae of Paragonimus in animal aquatic products and their products mainly live in the muscle tissue of crustaceans such as crabs, crickets, and macrobrachials.

Mash the sediment to separate or use pepsin to digest the animal host tissue after mashing to obtain metacercariae. Based on the morphological characteristics of Paragonimus metacercariae, preliminary Determine the type of metacercariae; by amplifying and sequencing the second internal transcribed spacer (ITS2) gene fragment of Paragonimus ribosomal DNA, the combined Identification of metacercariae of Geniminus.

3 Instruments and equipment

3.1 Biological microscope. 100x~400x.

3.2 Stereo microscope. 7.5x~150x.

3.3 PCR amplification instrument.

3.4 Gel imaging system.

3.5 Electrophoresis apparatus.

3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.

3.7 High-speed centrifuge. speed >=12000r/min.

3.8 Mesh screen. aperture 2mm (10 mesh); aperture 0.425mm (40 mesh).

3.9 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.

3.10 Conical measuring cup. 1000mL.

3.11 Glass beads. 425um~600um.

3.12 Ceramic mortar (diameter 16cm) and grinding rod.

3.13 Cyclotron oscillator.

4 Reagents and materials

4.1 Reagents

4.1.1 Hydrochloric acid. 36%~38% HCl solution.

4.1.2 Physiological saline. 0.85% NaCl solution.

4.1.3 1mol/LTris-HCl solution (pH8.0).

4.1.4 0.5mol/LEDTA solution (pH8.0).

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — all pages — is available in the English PDF.

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