GB 31610.4-2023National food safety standards - Inspection of animal aquatic products and their products for Clonorchis sinensis (English PDF)

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Issued by

National Health Commission of the People's Republic of China, State Administration for Market Regulation

Level / Type

National · Mandatory

Issue date

September 6, 2023

Implementation date

March 6, 2024

Scope

GB 31610.4-2023 (National food safety standards - Inspection of animal aquatic products and their products for Clonorchis sinensis) is available as an English-translated PDF.

GB 31610.4-2023 — test method. This standard is applicable to the inspection of Clonorchis sinensis metacercariae in animal aquatic products and their products.

Document preview — GB 31610.4-2023

National Standard of the People's Republic of China

Classification: X 09

Issued by: National Health Commission of the People's Republic of China, State Administration for Market Regulation

1 Scope
2 Principles
3 Instruments and equipment
3.1 Biological microscope. 100x~400x.
3.2 Stereo microscope. 7.5x~150x.
3.3 PCR amplification instrument.
3.4 Gel imaging system.
3.5 Electrophoresis apparatus.
3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.
3.7 High-speed centrifuge. speed >=12000r/min.
3.8 Mesh screen. aperture 0.25mm (60 mesh).
3.9 Conical measuring cup. 1000mL.
3.10 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.
4 Reagents and materials
4.1 Reagents

1 Scope

test method.

This standard is applicable to the inspection of Clonorchis sinensis metacercariae in animal aquatic products and their products.

2 Principles

Clonorchis sinensis metacercariae in animal aquatic products and their products mainly parasitize the muscle tissue of freshwater fish and freshwater shrimp. Observed by tabletting method Detect metacercariae or use pepsin to digest the muscle tissue of animal aquatic products and their products to obtain metacercariae. Based on the morphological characteristics of metacercariae, preliminary Determine the type of metacercariae. By amplifying and sequencing the second internal transcribed spacer (ITS2) gene fragment of the ribosomal DNA of Clonorchis sinensis, Identification of metacercariae of Clonorchis sinensis.

3 Instruments and equipment

3.1 Biological microscope. 100x~400x.

3.2 Stereo microscope. 7.5x~150x.

3.3 PCR amplification instrument.

3.4 Gel imaging system.

3.5 Electrophoresis apparatus.

3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.

3.7 High-speed centrifuge. speed >=12000r/min.

3.8 Mesh screen. aperture 0.25mm (60 mesh).

3.9 Conical measuring cup. 1000mL.

3.10 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.

4 Reagents and materials

4.1 Reagents

4.1.1 Hydrochloric acid. 36%~38% HCl solution.

4.1.2 Physiological saline. 0.85% NaCl solution.

4.1.3 1mol/LTris-HCl solution (pH8.0).

4.1.4 0.5mol/LEDTA solution (pH8.0).

4.1.5 10% SDS solution.

4.1.6 5mol/LNaCl solution.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — all pages — is available in the English PDF.

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