GB 31610.1-2023National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products (English PDF)

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Issued by

National Health Commission of the People's Republic of China, State Administration for Market Regulation

Level / Type

National · Mandatory

Issue date

September 6, 2023

Implementation date

March 6, 2024

Scope

GB 31610.1-2023 (National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products) is available as an English-translated PDF.

GB 31610.1-2023 — This standard specifies the morphological and PCR detection methods of Gnathostoma larvae in animal aquatic products and their products. This standard is applicable to the inspection of Gnatostoma larvae in animal aquatic products and their products.

Document preview — GB 31610.1-2023

National Standard of the People's Republic of China

Classification: X 09

Issued by: National Health Commission of the People's Republic of China, State Administration for Market Regulation

1 Scope
2 Principles
3 Instruments and equipment
3.1 Biological microscope. 100x~400x.
3.2 Stereo microscope. 7.5x~150x.
3.3 PCR amplification instrument.
3.4 Gel imaging system.
3.5 Electrophoresis apparatus.
3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.
3.7 Magnetic stirrer.
3.8 High-speed centrifuge. speed >=12000r/min.
3.9 Mesh screen. hole diameter 2mm (10 mesh).
3.10 Conical measuring cup. 1000mL.
3.11 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.
4 Reagents and materials
4.1 Reagents

1 Scope

This standard specifies the morphological and PCR detection methods of Gnathostoma larvae in animal aquatic products and their products.

This standard is applicable to the inspection of Gnatostoma larvae in animal aquatic products and their products.

2 Principles

Gnatostome larvae in animal aquatic products and their products mainly parasitize in the muscles and other tissues of freshwater fish, amphibians and other aquatic products.

The parasites are obtained by digesting animal host tissues with pepsin. According to the morphological characteristics of the larvae, the larvae of Gnatomostoma are judged. To detect suspicious parasites The gene fragment of the mitochondrial cytochrome C oxidase subunit I (cox1) of Gnathostomia nematode was over-amplified and sequenced to conduct the analysis of common Gnatomostoma larvae.

identification.

3 Instruments and equipment

3.1 Biological microscope. 100x~400x.

3.2 Stereo microscope. 7.5x~150x.

3.3 PCR amplification instrument.

3.4 Gel imaging system.

3.5 Electrophoresis apparatus.

3.6 Constant temperature incubator. 37 degrees C+/-1 degrees C.

3.7 Magnetic stirrer.

3.8 High-speed centrifuge. speed >=12000r/min.

3.9 Mesh screen. hole diameter 2mm (10 mesh).

3.10 Conical measuring cup. 1000mL.

3.11 Micropipette. 0.2uL~2.5uL, 1uL~10uL, 10uL~100uL, 100uL~1000uL.

4 Reagents and materials

4.1 Reagents

4.1.1 Hydrochloric acid. 36%~38% HCl solution.

4.1.2 Physiological saline. 0.85% NaCl solution.

4.1.3 1mol/LTris-HCl solution (pH8.0).

4.1.4 0.5mol/LEDTA solution (pH8.0).

4.1.5 10% SDS solution.

4.1.6 5mol/LNaCl solution.

4.1.7 3000U/mg pepsin.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — all pages — is available in the English PDF.

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