SN/T 5334.6-2020Digital PCR detection method for genetically modified plant products - Part 6: Genetically modified potatoes (English PDF)

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts.

--Part 1: General requirements and definitions;

--Part 2: Genetically modified soybeans;

--Part 3: Genetically modified corn;

--Part 4: Genetically modified rape;

--Part 5: Genetically modified cotton;

--Part 6: Genetically Modified Potatoes;

--Part 7: Genetically modified alfalfa;

--Part 8: Genetically modified sugar beet.

This document is part 6 of SN/T 5334-2020.

This document was drafted in accordance with the rules given in GB/T 1.1-2020.

Certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.

This document was proposed and managed by the General Administration of Customs of the People's Republic of China.

Drafting organizations of this document. Chinese Academy of Inspection and Quarantine Sciences, Qingdao Customs Technology Center of the People's Republic of China, People's Republic of China Heguo Dalian Customs Technology Center, Shenyang Customs Technology Center of the People's Republic of China.

1 Scope

This document stipulates the specific digital PCR (dPCR) quantitative detection method for the transgenic line AV43 in imported and exported potatoes.

This document is applicable to the quantitative detection of specific components of transgenic potato AV43 strains in potatoes. For the detection limits of this document, see the attachment Record A.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document.

SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1: General Requirements and Definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Main equipment and reagents

5.1 Endogenous gene and exogenous gene. the endogenous gene is potato beta sucrose invertase (fru.); the exogenous sequence is transgenic potato AV43 the 5 end of the strain's exogenous gene is combined with the potato endogenous genome. See Appendix A for primer and probe sequences.

5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

7 Judgment and expression of results

The results are expressed as follows.

- Both the internal standard gene and the foreign gene in the test result meet the negative and blank quality control requirements, indicating that no potato has been detected the endogenous gene fru, expressed as "potato ingredient not detected";

- In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said Genetically modified strains";

- In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that

8 Anti-pollution measures

The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix)