SN/T 5334.3-2020Digital PCR detection method for genetically modified plant products - Part 3: Genetically modified corn (English PDF)

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts.

--Part 1: General requirements and definitions;

--Part 2: Genetically modified soybeans;

--Part 3: Genetically modified corn;

--Part 4: Genetically modified rape;

--Part 5: Genetically modified cotton;

--Part 6: Genetically modified potatoes;

--Part 7: Genetically modified alfalfa;

--Part 8: Genetically modified sugar beet.

This document is part 3 of SN/T 5334-2020.

This document was drafted in accordance with the rules given in GB/T 1.1-2020.

1 Scope

This document specifies the digital PCR quantitative detection method for genetically modified strains in maize.

This document is applicable to the quantitative detection of 10 genetically modified strains in corn seeds and leaves. See Appendix A for the detection limits of this document.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document.

SN/T 5334.1 Digital PCR method for quantitative detection of genetically modified products Part 1: General requirements and definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Main equipment and reagents

5.1 Endogenous gene and exogenous gene. the endogenous gene is maize high mobility group proteins (HMG. high mobility group proteins);

The foreign genes are lines 98140, DAS40278-9, VCO-01981-5 and MON87427 insertion site 5'end cross-border sequence, and Strains BVLA430101, Bt799, IE034, 12-5, C0010.3.7 and C0030.3.5 insert the 3'-end cross-border sequence at the insertion site. Primers and See Appendix A for the probe sequence.

5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

7 Judgment and expression of results

See SN/T 5334.1 for calculation of results.

The results are expressed as follows.

- Both the internal standard gene and the foreign gene in the test result meet the negative and blank quality control requirements, indicating that no corn has been detected the endogenous gene Adh1, expressed as "no corn component detected";

- In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said Genetic strain";

- In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that

8 Anti-pollution measures

The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix) Primer probe sequence, reaction program and detection limit