SN/T 5334.2-2020Digital PCR Detection Method of Genetically Modified Plant Products - Part 2: Genetically Modified Soybean (English PDF)

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts.

--Part 1: General requirements and definitions;

--Part 2: Genetically modified soybeans;

--Part 3: Genetically modified corn;

--Part 4: Genetically modified rape;

--Part 5: Genetically modified cotton;

--Part 6: Genetically Modified Potatoes;

--Part 7: Genetically modified alfalfa;

--Part 8: Genetically modified sugar beet.

This document is part 2 of SN/T 5334-2020.

This document was drafted in accordance with the rules given in GB/T 1.1-2020.

1 Scope

This document specifies the quantitative detection method of genetically modified strains specific to imported and exported soybeans by digital PCR (dPCR).

This document is applicable to the quantitative detection of genetically modified components in soybean seeds and leaves. See Appendix A for the detection limits of this document.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document.

SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1: General Requirements and Definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Main equipment and reagents

5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

7 Judgment and expression of results

Refer to SN/T 5334.1 for the calculation method of the results.

The results are expressed as follows.

- In the test results, both the internal standard gene and the foreign gene meet the negative and blank quality control requirements, indicating that no soybean has been detected.

Source gene Lectin, expressed as "no soybean component detected";

- In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said Genetic strain";

- In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that

8 Anti-pollution measures

The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.