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SN/T 3504-2013Quarantine protocol for metacercaria of Paragonimus in crustaceans (English PDF)

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

March 1, 2013

Implementation date

September 16, 2013

Scope

SN/T 3504-2013 (Quarantine protocol for metacercaria of Paragonimus in crustaceans) is available as an English-translated PDF.

SN/T 3504-2013 - This standard specifies the paragonimiasis morphological analysis and PCR detection method metacercariae. This standard applies to crabs, shrimp and other crustaceans and aquatic Paragonimus metacercariae quarantine, epidemiological investigation and monitoring.

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Document preview — SN/T 3504-2013

National Standard of the People's Republic of China

ICS: 07.080
Classification: B41

Issued by: General Administration of Customs

Foreword
1 Scope
2 Normative references
3 Overview
4 Equipment and Materials
4.1 Instruments and equipment
4.2 Reagents and solutions

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Chinese Center for Disease Control Parasitic Diseases Control and Prevention the system, Guangxi University.

1 Scope

This standard specifies the paragonimiasis morphological analysis and PCR detection method metacercariae.

This standard applies to crabs, shrimp and other crustaceans and aquatic Paragonimus metacercariae quarantine, epidemiological investigation and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling

3 Overview

Commonly known as lung fluke Paragonimus mainly parasitic in humans, dogs, cats, rats and other mammals lungs. People from ingestion of raw, undercooked containing Paragonimus metacercariae crabs, clam mantis prawns and other aquatic products and infection. Paragonimus classification, distribution and life history, see Appendix A.

4 Equipment and Materials

4.1 Instruments and equipment

4.1.1 Biological microscope.

4.1.2 stereomicroscope.

4.1.3 PCR amplification.

4.1.4 electrophoresis.

4.1.5 gel imaging system.

4.1.6 Mesh sieve (10 mesh, 40 mesh)

4.1.7 glass beads (425µm ~ 600µm).

4.2 Reagents and solutions

4.2.1 Taq enzyme (5U/µL).

4.2.2 2.5mmol/LdNTP.

4.2.3 25mmol/LMgCl2.

4.2.4 10 × PCR buffer (without Mg2).

4.2.5 Molecular Weight indicator. 100bp ~ 2000bpDNAmarker.

4.2.6 water (in line with GB/T 6682 in a water regulations).

4.2.7 artificial digestive juice, Tris- acetate electrophoresis buffer (TAE), agarose gel, 6 × loading buffer (preparation see Appendix B).

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 8 pages — is available in the English PDF.

Referenced standards

Normative references

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