SN/T 3497-2013Quarantine protocol for Gnathostoma in aquatic products (English PDF)
This is a limited preview
Buy now to download the full PDF (9 pages)
Need quotation for your management?
Get a formal quotation for SN/T 3497-2013 in 30 minutes.
Issued by
General Administration of Customs
Level / Type
National · Recommended
Issue date
March 1, 2013
Implementation date
September 16, 2013
Scope
SN/T 3497-2013 (Quarantine protocol for Gnathostoma in aquatic products) is available as an English-translated PDF.
SN/T 3497-2013 - This standard specifies the morphological analysis and PCR detection method jaw spinigerum third stage larvae. This standard applies to fish in the jaw spinigerum quarantine, epidemiological investigation and monitoring.
Found what you need?
Document preview — SN/T 3497-2013
National Standard of the People's Republic of China
Issued by: General Administration of Customs
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Overview
- 4 Equipment and Materials
- 4.1 Instruments and equipment
- 4.2 Reagents and solutions
- 4.3 Primer
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Guangxi University, Guangxi Zhuang Autonomous Region Disease Prevention and Control System Center, Chinese Center for Disease Control Parasitic Diseases Control and Prevention, Heilongjiang People's Republic of China Exit Inspection and Quarantine.
1 Scope
This standard specifies the morphological analysis and PCR detection method jaw spinigerum third stage larvae.
This standard applies to fish in the jaw spinigerum quarantine, epidemiological investigation and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Overview
Jaw nematode (Gnathostomiasis) is a human beast from the jaw spinigerum (Gnathostoma) caused by parasitic disease. Mainly due to people Ingestion of raw or undercooked containing jaw spinigerum third stage larvae of aquatic worm disease caused by jaw mouth. Jaw spinigerum classification, life history, points Cloth See Appendix A.
4 Equipment and Materials
4.1 Instruments and equipment
4.1.1 Biological microscope.
4.1.2 stereomicroscope.
4.1.3 PCR amplification.
4.1.4 electrophoresis.
4.1.5 gel imaging system.
4.1.6 mesh sieve (10 mesh).
4.2 Reagents and solutions
4.2.1 Taq enzyme (5U/µL).
4.2.2 2.5mmol/LdNTP.
4.2.3 25mmol/LMgCl2.
4.2.4 10 × PCR buffer (without Mg2).
4.2.5 Molecular Weight indicator. 100bp ~ 2000bpDNAmarker.
4.2.6 50% and 70% ethanol.
4.2.7 water, in line with GB/T 6682 stipulated a water.
4.2.8 Artificial digestion, phenol transparent lactate solution, Tris-acetate electrophoresis buffer (the TAE), agarose gel, 6 × loading buffer (preparation See Appendix B).
4.3 Primer
Amplification jaw spinigerum ribosomal DNA second internal transcribed spacer (ITS2) primer sequences are.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 9 pages — is available in the English PDF.
Referenced standards
How to Download SN/T 3497-2013
- 1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
- 2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
- 3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
- 4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.
Related Standards
GB/T 18088-2026 — Sampling for entry and exit animal quarantine
GB/T 6682-2008 — Water for analytical laboratory use - Specification and test methods
SN/T 0330-2025 — General rules for microbiological examinations of export food
Secure payment via Stripe
Payments accepted
SN/T 3497-2013
$269.00