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SN/T 3199-2012Quarantine protocol for Pasteurellosis in rabbit (English PDF)

兔巴氏杆菌病检疫技术规范

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

October 23, 2012

Implementation date

May 1, 2013

Scope

SN/T 3199-2012 is the English-translated version of 兔巴氏杆菌病检疫技术规范.

SN/T 3199-2012 - This standard specifies the rabbit pasteurellosis sample preparation techniques after laboratory testing and quarantine treatment. This standard applies to rabbit pasteurellosis quarantine.

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Document preview — SN/T 3199-2012

National Standard of the People's Republic of China

Issued by: General Administration of Customs

Foreword
1 Scope
2 Normative references
3 Disease Overview
4 Initial diagnosis
5.1 Material
5.2 Isolation

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Jiangxi Exit Inspection and Quarantine, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau of Henan Agricultural University.

1 Scope

This standard specifies the rabbit pasteurellosis sample preparation techniques after laboratory testing and quarantine treatment.

This standard applies to rabbit pasteurellosis quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents GB 16548 diseases and diseases of animals and animal products processing biosafety regulations

3 Disease Overview

See Appendix A.

4 Initial diagnosis

A reference in Appendix clinical symptoms and pathological changes make a preliminary diagnosis.

Isolation and Identification of 5

5.1 Material

5.1.1 Reagents. Ammonium oxalate crystal violet, Gram iodine solution, 95% alcohol, carbonic acid fuchsin, methanol, Reiter's dye, methylene blue dye, 10% sheep Whole blood, red blood cells of healthy animals 5.1.2 1% peptone water, preparation methods, see Appendix B, B.1.

5.1.3 serum broth, preparation methods, see Appendix B, B.2.

5.1.4 Mading Shi broth, preparation methods, see Appendix B, B.3.

5.1.5 10% blood agar, preparation methods, see Appendix B B.4.

5.1.6 MacConkey agar, preparation methods, see Appendix B in B.5.

5.1.7 indole test reagent preparation methods, see Appendix B B.6.

5.1.8 oxidase test reagent preparation methods, see Appendix B B.7.

5.1.9 sugar fermentation medium, method of preparation see Appendix B B.8.

5.1.10 motility medium, method of preparation see Appendix B B.9.

5.2 Isolation

5.2.1 Diseased acquisition

5.2.1.1 The most acute and acute cases only collect dead rabbit liver, spleen, heart; chronic cases collecting local tissue lesions; for not fresh or

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 11 pages — is available in the English PDF.

Referenced standards

Normative references

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