SN/T 1477-2012Determination of paclobutrazol residues in food for export (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

Instead of the standard SN/T 1477-2004 "import and export food paclobutrazol residues in" this standard compared with SN/T 1477-2004, the main technical changes are as follows.

--- Remove the sampling step;

--- Homemade Florisil column to column commercialization;

--- Modify the lower limit of detection.

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This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. Chinese Academy of Inspection and Quarantine.

1 Scope

This standard specifies the MET residues in food for export of gas chromatography - mass spectrometry.

This standard is applicable to the export of rice, pears paclobutrazol residues determination.

2 Method summary

With acetone or ethyl acetate - acetone mixture to extract the sample remaining MET, Florisil solid-phase extraction (SPE) and gas chromatography -Mass spectrometry (GC-MS) detection, external standard.

3 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.

3.1 acetone. pesticide grade.

3.2 hexane. pesticide grade.

3.3 ethyl acetate. pesticide grade.

3.4 ethyl acetate - acetone mixture. Take 20mL acetone, diluted to 100mL with ethyl acetate.

3.5 eluent (n-hexane - acetone mixture). Take 5mL acetone, diluted to 100mL with n-hexane.

3.6 eluent (n-hexane - acetone mixture). Take 15mL acetone, diluted to 100mL with n-hexane.

3.7 MET standard substance. purity >=99%.

3.8 MET standard solution. Accurately weigh an adequate amount of MET standard substance (accurate to 0.1mg), dissolved in a small amount of acetone, hexane Preparation of a concentration of 100µg/mL of the standard stock solution. Diluted to concentrations applicable standard working solution with n-hexane as needed.

3.9 anhydrous sodium. 650 °C burning 4h, placed in the dryer to save.

3.10 Florisil SPE column. 2g, 12mL.

4 Instruments and equipment

4.1 Gas chromatography - mass spectrometry. equipped with an electronic bombardment source.

4.2 rotary evaporator.

4.3 oscillator.

4.4 conical flask. a grinding gag, 250mL.

4.5 pear-shaped bottle. 250mL.

5 Sample preparation and preservation