LY/T 3086.2-2019Technical regulation for ex situ conservation of wild plants with extremely small populations (English PDF)

3.1 Ex situ conservation

Protect extremely small populations of wild plants outside their native habitat.

3.2 Gene library

DNA fragments containing different genes of a certain organism are introduced into a population of recipient bacteria for storage, and each recipient bacteria contains Different genes.

3.3 Potential distribution area

Species are not yet distributed, but are suitable for all possible areas for their survival and reproduction.

3.4 Microenvironment

Adjacent environments that have a direct impact on living beings refer to specific habitats within a small area.

4 Basis, principles and objectives of ex situ conservation of very small populations of wild plants

4.1 Basis for ex situ protection

(1) Severe degradation in situ;

(2) The number of individuals is lower than the minimum viable population, and the population is difficult to maintain;

(3) Sudden changes in living conditions face a serious survival crisis.

4.2 Principles of ex situ protection

(1)Do not damage the native population and its habitat;

(2) Establish as many relocation protection sites as possible when conditions are available;

(3) Try to save multiple individuals and genotypes at each ex-situ conservation site;

(4) Try to adopt multiple ways to carry out relocation protection;

(5) Fully consider the role of artificial regulation in ex-situ conservation.

4.3 Objectives of ex situ conservation

Preserving wild plant germplasm resources of very small populations, reducing the risk of extinction in situ, increasing the probability of population reproduction and eventually restoring wild species group.

5.1 Ex-situ conservation sites

Utilize existing botanical gardens, arboretums or other cultivated lands to protect the relocated plants after natural or artificial propagation.

Set up a special base of very small populations of wild plants or other rare and endangered plants for ex-situ conservation.

5.2 Relocation protection methods

(1) The whole plant is preserved;

(2) Preservation of seeds and tissues;

(3)Gene library preservation.

6.1 Location selection

(1) Select a site with similar conditions to in situ climate, soil, and organisms (including mycorrhizal fungi, pollinating insects, etc.) as the ex-situ conservation base Ground (2) According to the potential distribution area of the species and the actual conditions, select a suitable site as the ex-situ protection base;

(3) When comprehensively considering the needs and conditions of work such as protection and research, take control measures to artificially create a suitable small environment.

Select some sites as relocation protection bases.

6.2 Land preparation

For land preparation technology, refer to GB GB/T 15776-2016.

6.3 Nursery

Refer to GB/T 6001-85 for nursery technology.

6.4 Seedling preparation

Container seedlings should be used to ensure intact root systems and later seedling survival rates.

6.5 Seedling transportation and quarantine

Seedlings must be transported with a special soil truck, and should be transported at an appropriate time (such as before budding in early spring or after falling leaves).

Show quarantine of diseases and insect pests. Specific quarantine procedures can refer to SN/T 1157-2014.

6.6 Seedling planting

Dig the planting holes 1-2 days before planting and supplement the base fertilizer. During the cultivation process, ensure that the root system of the seedlings is stretched, and the soil is firmly grounded.

6.7 Seedling Management

Watering, fertilizing, weeding, pest control, shading, timely removal of weeds and other competing plants, specific management of seedlings after planting You can refer to GB GB/T 15776-2016.

7.1 Construction of species and habitat information database and archive management

Establish a database and archives management system for relevant information such as wild plant species, habitats and conservation status of the extinct small populations.

7.2 Relocation of small environment

According to the physiological and ecological needs of the wild plants at different growth stages in small populations, the management of shading, weeding, watering and fertilizing is implemented Measures to artificially regulate the small environment for plant growth and development, to maintain the normal growth and development of ex-situ plants.

7.3 Pest and disease control

The pests and diseases of ex-situ plants should be biologically or chemically controlled as soon as they are discovered.

7.4 Relocation protection monitoring

Each plant individual in the ex-situ conservation population must be listed and numbered, and the growth, reproduction, and regeneration status of the ex-situ plants should be monitored regularly.

Environmental factors such as microclimate and soil.

8.1 Short-term standards

According to GB/T 15776-2016, survival rate from seedlings (below 40%, poor; 40-80%, medium; above 80%, excellent), growth status (Good, normal, poor), preservation rate (below 20%, poor; 20-70%, medium; more than 70%, excellent) and reproduction status (normally firm, not strong) for comprehensive evaluation.

8.2 Long-term standards

The process of ex situ protected plants capable of producing fertile seeds or other progeny propagules to achieve a complete life history is defined as the ex situ conservation is completely successful, and can only grow normally but not reproduce. The offspring is defined as part of the ex situ conservation, which cannot grow normally or grows poorly.

It is defined as the unsuccessful protection of ex-situ conservation, while the failure to grow at all is defined as the unsuitable protection of ex-situ conservation.