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HY/T 147.3-2013Code of practice for marine monitoring technology. Part 3: Organism (English PDF)

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Issued by

State Oceanic Administration

Level / Type

National · Recommended

Issue date

April 25, 2013

Implementation date

May 1, 2013

Scope

HY/T 147.3-2013 (Code of practice for marine monitoring technology. Part 3: Organism) is available as an English-translated PDF.

HY/T 147.3-2013 — This part of HY/T 147 specifies the analysis methods for monitoring items of marine organisms such as fish, shrimp, and shellfish. This part is applicable to the monitoring of marine life in the open sea, offshore sea and estuary.

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Document preview — HY/T 147.3-2013

National Standard of the People's Republic of China

ICS
07.060
Classification
A 45

Issued by: State Oceanic Administration

Contents

  • Preface
  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 General provisions
  • 5 Determination of total mercury---thermal decomposition cold atomic absorption spectrometry
  • 6 Simultaneous determination of copper, lead, zinc, cadmium, chromium, manganese, nickel, arsenic, aluminum, and iron---inductively coupled plasma mass spectrometry
  • 7 Polycyclic aromatic hydrocarbons
  • 8 Phthalates
  • 9 Determination of Organophosphorus Pesticides---Gas Chromatography
  • 10 Determination of organotin---gas chromatography
  • 11 Determination of polybrominated diphenyl ethers---gas chromatography/mass spectrometry
  • Appendix A (Normative Appendix) Record Sheet
  • Appendix B (informative appendix) Method detection limit
  • Appendix C (informative appendix) Determination of EROD---kinetic fluorescence method
  • Appendix D (informative appendix) Determination of total acetylcholinesterase in marine organisms

Foreword

HY/T 147 "Marine Monitoring Technical Regulations" is divided into seven parts.

---Part 1: Sea water;

---Part 2: Sediment;

---Part 3: Organisms;

---Part 4: Ocean atmosphere;

---Part 5: Marine ecology;

---Part 6: Marine Hydrology, Meteorology and Sea Ice;

---Part 7: Satellite remote sensing technology methods.

This part is part 3 of HY/T 147.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

This part was proposed by the National Marine Environmental Monitoring Center.

This part is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283).

This section is responsible for the drafting unit. National Marine Environmental Monitoring Center.

Participating in the drafting of this section. South China Sea Environmental Monitoring Center of State Oceanic Administration, East China Sea Environmental Monitoring Center of State Oceanic Administration, State Oceanic Administration Beihai Environmental Monitoring Center.

1 Scope

This part of HY/T 147 specifies the analysis methods for monitoring items of marine organisms such as fish, shrimp, and shellfish.

This part is applicable to the monitoring of marine life in the open sea, offshore sea and estuary.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document.

GB/T 6379.2-2004 Measurement methods and accuracy of results (accuracy and precision) Part 2: Determine standard measurement methods Basic method of method repeatability and reproducibility GB 17378.1 Marine Monitoring Specification Part 1: General GB 17378.2 Marine Monitoring Specification Part 2: Data Processing and Analysis Quality Control GB 17378.3 Marine Monitoring Specification Part 3: Sample Collection, Storage and Transportation GB 17378.6 Marine Monitoring Specification Part 6: Biological Analysis

3 Terms and definitions

The following terms and definitions defined in GB 17378.1 and GB 17378.2 apply to this document.

Marine biological sample Marine organisms in the open sea, offshore waters and estuaries, including fish, shrimp, shellfish and other marine organisms.

Freeze-drying

A drying method in which the material to be dried is quickly frozen, and then the ice in it is sublimated into water vapor under high vacuum conditions. Due to ice the sublimation takes away the heat to keep the freeze-drying process in a low-temperature frozen state, which is conducive to retaining the activity of some biological samples (such as proteins).

4 General rules

4.1 Sample collection

The collection, preparation, storage, transportation and pretreatment of samples shall be carried out in accordance with the relevant regulations of GB 17378.3 and GB 17378.6.

4.2 Reagents and materials

The experimental supplies of this part should be handled according to the following requirements.

a) Test tubes with scales, concentrate bottles, pipettes, volumetric flasks, etc. for experiments should be calibrated before use;

b) Glass containers and utensils should be rinsed with water, washed with detergent, then washed with ultrapure water, dried or dried naturally;

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 80 pages — is available in the English PDF.

Referenced standards

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