GB/Z 156-2025Good practice for dynamic light scattering(DLS) measurements (English PDF)
动态光散射法（DLS）测量操作指南
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 3, 2025
Implementation date
December 3, 2025
Scope
GB/Z 156-2025 is the English-translated version of 动态光散射法（DLS）测量操作指南.
GB/Z 156-2025 is a guiding technical document, an identical adoption of ISO/TR 22814:2020, on good practice in dynamic light scattering measurements. It supplements GB/T 29022-2021 and covers what information to gather before analysis, whether a sample suits DLS at all, sample preparation, checking that the instrument works, and how to judge and read the data. Data quality and interpretation are treated separately for correlation function processing and for frequency power spectrum processing, and a clause addresses method robustness. It also discusses how the scattering angle changes practice, for instance filtering out large particles and discarding the first drops of sample when measuring at a forward angle. It is written to help users plan measurements of solid particles, emulsions and bubbles, and keeps to practical steps rather than theory. It was issued on 3 December 2025.
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Document preview — GB/Z 156-2025
National Standard of the People's Republic of China
- ICS
- 19.120
- Classification
- A 28
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Preface
- Introduction
- 1.Scope
- 2 Normative References
- 3.Terms and Definitions
- 4.Instrument Type
- 4.1 Overview
- 4.2 Information before analysis
- 4.3 Sample Suitability for DLS Analysis
- 4.4 Sample Preparation
- 4.5 Instrument Calibration
- 4.6 Data Quality and Interpretation - Correlation Analysis
- 4.7 Data Quality and Interpretation - Frequency Power Spectrum Analysis
- 4.8 Method Robustness
- Reference
Foreword
This document is a report-type guidance technical document.
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents". Drafting.
This document is equivalent to ISO /T R22814.2020 "Guide to Operation of Dynamic Light Scattering (DLS) Measurements", and the document type is changed from ISO technical standard.
The technical report has been adjusted to become a national standardization guiding technical document in my country.
Introduction
Dynamic light scattering (DLS) is a widely used characterization technique for particles with equivalent hydrodynamic diameters in the submicron range. Modern instruments allow... While some users may have received limited training or lack background knowledge, the problem is that not all users are familiar with the technology.
The potential flaws and limitations of the results, and how to interpret them correctly.
Therefore, this document is formulated as an operational guide for dynamic light scattering measurements and as part of GB/T 29022-2021 "Dynamic Particle Size Analysis".
1 Scope
This document provides a guide to measurement and result interpretation using dynamic light scattering (DLS) technology, outlining how to perform measurements appropriately.
Information such as sample preparation methods, how to verify instrument operation, and how to correctly interpret data (including data quality assessment methods) is extensive.
GB/T 29022-2021 provides guidelines for measurement.
This document is intended to assist users in planning experiments for measurements of solid particles, emulsions, and bubbles, particularly regarding how to obtain the necessary samples.
For information and to determine whether DLS is the most suitable detection method, the focus should be on the practical operations required to obtain high-quality DLS measurement results.
It focuses on steps rather than theoretical discussions.
2 Normative references
This document has no normative references.
3 Terms and Definitions
This document does not contain any terms or definitions that need to be defined.
4 Instrument Types
4.1 Overview
Discussing best operating guidelines requires understanding the type of instrument used. Different optical configurations require different adjustment methods to control light transmission.
Different signal processing techniques require different methods to adjust background conditions; different analysis techniques require different conditions of the signal being processed.
Parameters. Two commonly used types are zero-error detection using correlation function processing (see 9.2 of GB/T 29022-2021) and using frequency... Heterodyne detection for power spectrum processing (see 9.3 of GB/T 29022-2021).
Furthermore, the best operating guidelines related to the instrument type also depend on the scattering angle used for the measurement. For example, large particle impurities typically produce... Forward scattering at a large angle is stronger than that at a large angle. Therefore, when measuring a sample using the forward scattering angle, it is usually necessary to carefully observe the sample before adding it.
The cleanliness of the cuvettes is ensured, large particle sizes are filtered out, and only the particle size distribution (PSD) of interest in the sample is retained. The injection filter is then discarded.
The first few drops of sample are used to remove filter residue. Furthermore, when the scattering angle is known to be close to 180°, higher-order scattering from high-concentration samples can be well observed.
Since the relaxation time is approximately equal to that of a single scattering event, backscattering can be used to characterize high-concentration media, provided that the volume scattering loss can be avoided.
Sample density. Many commercial instruments use optical, mechanical, or optomechanical means to move the optical detection point closer to the cuvette wall.
4.2 Information before analysis
4.2.1 Sample Information
Customers or submitters of DLS-analyzed samples should provide as much information as possible related to the sample measurements. A lack of information does not preclude analysis.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 18 pages — is available in the English PDF.
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