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GB/T 45137-2024Technical code of practice with genome editing and breeding for livestock and poultry (English PDF)

畜禽基因组编辑育种技术规程

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

December 31, 2024

Implementation date

December 31, 2024

Scope

GB/T 45137-2024 is the English-translated version of 畜禽基因组编辑育种技术规程.

GB/T 45137-2024 is the Chinese national standard covering breeding livestock and poultry from animals whose genome has been edited with CRISPR/Cas — the biosafety level the laboratory has to meet, the training the operators need, the guide RNA and the target sequence that decide where the cut falls, the vector and the editing steps, the records kept on materials, sequences and result judgement, and the animal welfare rules that apply because the work ends in a live animal. First edition. In force from 31 December 2024. Issued on 31 December 2024, it has been in force since 31 December 2024.

Document preview — GB/T 45137-2024

National Standard of the People's Republic of China

ICS
65.020.30
Classification
B 43

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 3.1 Genome editing
  • 3.2 guide RNA guideRNA;gRNA
  • 4 Requirements
  • 4.1 Experimental site
  • 4.2 Personnel
  • 4.3 Records
  • 4.4 Animal Welfare

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the China National Institute of Standardization.

This document was drafted by: Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, China Agricultural University, Northwest Agriculture and Forestry University, Inner Mongolia University, Jilin University, China National Institute of Standardization.

The main drafters of this document are: Ao Hong, Li Kui, Mou Yulian, Liu Zhiguo, Lian Zhengxing, Zhang Yong, Li Guangpeng, Ouyang Hongsheng, Wang Bingyuan and Ma Aijin.

Technical regulations for livestock and poultry genome editing breeding

1 Scope

This document establishes the technical process for genome editing breeding of livestock and poultry and stipulates relevant requirements and operational procedures.

This document applies to CRISPR/Cas technology operations for livestock and poultry genome editing breeding.

2 Normative references

GB 19489

3 Terms and definitions

The following terms and definitions apply to this document.

3.1 Genome editing

Genetic engineering that targets and modifies specific deoxyribonucleic acid (DNA) fragments in the genome of an organism without introducing foreign DNA technology.

3.2 guide RNA guideRNA;gRNA

Small molecule ribonucleic acid (RNA) plays a guiding and template role in genome editing technology and can mediate the genome editing process.

4 Requirements

4.1 Experimental site

Laboratory requirements should comply with the corresponding biosafety level requirements of GB 19489.

4.2 Personnel

Necessary training in experimental techniques and biosafety management standards should be obtained.

4.3 Records

It should include but not be limited to the start and end time of the test, the name, source, number, storage information of the test material, target gene sequence information information, carrier structure, test spectrum and result judgment, etc.

Records should be kept standardized, complete and consistent, and relevant regulations should be made for the preservation of records.

4.4 Animal Welfare

The requirements of relevant animal welfare regulations should be met.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Editions of GB/T 45137

EditionTitleRevisionStatus
GB/T 45137-2024Technical code of practice with genome editing and breeding for livestock and poultrycurrent editionCurrent

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