Valid

GB/T 44640-2024Chemicals - Xenopus eleutheroembryonic thyroid assay (English PDF)

化学品非洲爪蟾胚胎甲状腺活性试验

Preview PDF

This is a limited preview

Buy now to download the full PDF (39 pages)

Need quotation for your management?

Get a formal quotation for GB/T 44640-2024 in 30 minutes.

Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

September 29, 2024

Implementation date

April 1, 2025

Scope

GB/T 44640-2024 is the English-translated version of 化学品非洲爪蟾胚胎甲状腺活性试验.

GB/T 44640-2024 is the Chinese standard "Chemicals - Xenopus eleutheroembryonic thyroid assay". Its scope clause reads: This document describes a method for the thyroid activity assay in Xenopus laevis embryos.

This document is suitable for screening the response of African Xenopus embryos to potential thyroid-active substances; suitable for screening potential thyroid-active substances.

This document does not apply to volatile chemicals; does not apply to chemicals that accumulate in African clawed frog embryos and are emitted at wavelengths of 450nm~ Chemical substances that emit fluorescence in the wavelength range of 500nm~550nm when excited in the range of 500nm.

Found what you need?

Document preview — GB/T 44640-2024

National Standard of the People's Republic of China

ICS: 13.300
Classification: A80

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
1 Scope
2 Normative references
3 Terms and definitions, abbreviations
4 Test Principle
5 Test substance information
6 Reference
7 Method Description
8 Test Procedure
9 Quality Assurance and Quality Control
10 Data analysis and test result evaluation
Appendix A (Normative) Overview of test conditions
Appendix B (normative) Calibration. Determination of the optimal settings for fluorescence spectrometers
Appendix C (Informative) NF45 and NF47 embryos
Appendix D (Normative) Receiving embryos. adaptation and batch acceptance
Appendix E (Informative) Breeding
Appendix F (informative) Some physical and chemical properties of dilution water
Appendix G (Informative) Photographic Guidelines for Identifying Normal and Abnormal Embryos
Appendix H (Informative) Embryo Sorting. Abnormal Pigmentation
Appendix I (Informative) Sample 6-well plate setup
Appendix J (Informative) 96-well plate fluorescence reading protocol
Appendix K (Informative) Embryo Positioning
Appendix L (Informative) Data Statistical Analysis Methods

1 Scope

This document describes a method for the thyroid activity assay in Xenopus laevis embryos.

This document is suitable for screening the response of African Xenopus embryos to potential thyroid-active substances; suitable for screening potential thyroid-active substances.

This document does not apply to volatile chemicals; does not apply to chemicals that accumulate in African clawed frog embryos and are emitted at wavelengths of 450nm~

Chemical substances that emit fluorescence in the wavelength range of 500nm~550nm when excited in the range of 500nm.

2 Normative references

The contents of the following documents constitute essential clauses of this document through normative references in this document.

For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to

This document.

GB/T 21801 Respirometric test for rapid biodegradability of chemicals

GB/T 21802 Improved MITI test for the rapid biodegradability of chemicals (I)

GB/T 21803 DOC reduction test for rapid biodegradability of chemicals

GB/T 21831 Ready biodegradability of chemicals. Closed bottle test

GB/T 21845 Test for water solubility of chemicals

GB/T 21852 Chemical Partition Coefficient (n-octanol-water) High Performance Liquid Chromatography Test

GB/T 21853 Partition coefficient of chemicals (n-octanol-water) Shake bottle test

GB/T 21855 Test for hydrolysis of chemicals in relation to pH

GB/T 21856 Carbon dioxide generation test for readily biodegradable chemicals

GB/T 21857 Improved OECD screening test for the ready biodegradability of chemicals

GB/T 22052 Method for determining the relationship between vapor pressure and temperature and initial decomposition temperature of liquids using a liquid vapor pressure gauge

GB/T 22228 Determination of the vapor pressure of solids and liquids of industrial chemicals in the range of 10-1Pa to 105Pa Static method

GB/T 22229 Determination of vapor pressure of solid and liquid chemicals for industrial use in the range of 10-3Pa to 1Pa

Balance

GB/T 27850 General rules for rapid biodegradability of chemicals

GB/T 27861 Chemicals - Acute toxicity test for fish

GB/T 29763 Chemicals - Acute toxicity test for rare goby

GB/T 30664 Chemical amphibian metamorphosis test

GB/T 42426 Chemical vapor pressure test

DevelopmentAssay)

CO2 in Sealed Vessels)

ChemicalsinWater-DirectPhotolysis) […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 39 pages — is available in the English PDF.

Referenced standards

Normative references

Similar standards

GB 38031-2025|GB/T44640-2024|GB/T 1.1-2020|GB/T 21801|GB/T 21802|GB/T 21803|GB/T 21831|GB/T 21845

How to Download GB/T 44640-2024

1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.